Because of its integration and miniaturization, microfluidic chips can greatly shorten processing time, improve detection resolution and accuracy, and reduce consumption and cost. In biological and medical research, the separation of different types of cells has always been an important field of cell research, which can bring great convenience to the diagnosis and treatment of many diseases, but also will bring breakthroughs in life science research. Using single cell analysis technology to detect chemical components in a single cell is helpful to understand basic cell functions and to detect and identify a small number of abnormal cells in a large cell population. The research of cell biology is an important driving force for the research of modern life science. Accurate and effective cell biology research experiments enable human beings to further understand the generation and development process of life structure. In this paper, the behavior of fluid and cells in microfluidic chips is investigated. At the same time, the method of driving cells by high-focus laser microbeam is explored theoretically and experimentally.