Protective Effect of Melatonin on Oxidative Stress in Hippocampus of Schizophrenic Rats

Xianghong Gao ； Jinjin Mou ； Peilun Xiao ； Lijie Zhang ； Xiaohua Zhao

《World Scientific Research Journal》 6卷11期 (2020/11) Pp. 157-162

https://doi.org/10.6911/WSRJ.202011_6(11).0020

摘要

Objective: To investigate the protective effect of melatonin (MT) on oxidative stress of hippocampal neurons in schizophrenic rats. Methods: 36 male SD rats were divided into 3 groups: control group, model group and model +MT group (10mg/kg), with 12 rats in each group. The rat model of schizophrenia was established by intraperitoneal injection of MK-801. After successful modeling, the model +MT group was intraperitoneally injected with MT, while the control group and the model group were injected with the same dose of 10% alcohol for 14 days. Morris water maze experiment was used to detect the learning and memory function of rats. Primary hippocampal cells were cultured by disconnection. The contents of ROS, SOD and MDA related factors in the culture medium of hippocampal cells were detected by kit method, and the number of apoptosis of hippocampal neurons was detected by TUNEL method. Results: Compared with the control group, the learning and memory function of the rats in the model group was impaired, the contents of ROS and MDA in the hippocampus were significantly increased, SOD activity was decreased, and the number of apoptotic neurons was significantly increased (P ＜ 0.05).Compared with the model group, the learning and memory functions of the rats in the model +MT group were significantly improved, the contents of ROS and MDA in the hippocampus were decreased, SOD activity was increased, and the number of apoptotic neurons was significantly decreased (P＜0.05). Conclusion: MT can significantly reduce the oxidative stress response and protect the neurons in the hippocampus of schizophrenic rats.

關鍵字

Schizophrenia ； Melatonin ； hippocampal ； oxidative stress

參考文獻

MA D, GUESTP C. Generation of the acute phencyclidine rat model for proteomic studies of schizophrenia[J]. Adv Exp Med Biol,2017,97(4):257-261.

ANDERSON G, MAES M. Oxidative/nitrosative stress and immuno-inflammatory pathways in depression: treatment implications[J]. Curr Pharm Des, 2014, 20(23): 3812-3847.

Paradies G, Paradies V, Ruggiero FM, et al.Protective role of melatonin in mitochondrial dysfunction and relater disorders[J]. Arch Toxicol, 2015, 89(6): 923-939.

Liu Ting, after all, Wang pan, et al. MAPK / ERK signaling pathway participates in neuroprotective effect of melatonin on cerebellum of Alzheimer's disease rats [J]. Chinese Pharmacological Bulletin, 2019, 35 (3): 402-407

Wan Hongjiao, he Huan, Liu Shenghui, et al. Effect of Wendan Decoction on NRG1 and ErbB4 protein expression in hippocampus of rats with schizophrenia [J]. Chinese medicine pharmacology and clinic, 2016, 32 (3): 12-16