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Kaempferia parviflora rhizome extract and Myristica fragrans volatile oil increase the levels of monoamine neurotransmitters and impact the proteomic profiles in the rat hippocampus: Mechanistic insights into their neuroprotective effects
摘要
Potentially useful in the treatment of neurodegenerative disorders, Kaempferia parviflora and Myristica fragrans have been shown to possess a wide spectrum of neuropharmacological activities and neuroprotective effects in vivo and in vitro. In this study, we determined whether and how K. parviflora ethanolic extract and M. fragrans volatile oil could influence the levels of neurotransmitters and the whole proteomic profile in the hippocampus of Sprague Dawley (SD) rats. The effects of K. parviflora and M. fragrans on protein changes were analyzed by two-dimensional gel electrophoresis (2D-gel), and proteins were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). The target proteins were then confirmed by Western blot. The levels of neurotransmitters were evaluated by reversed-phase high-performance liquid chromatography (RP-HPLC). The results showed that K. parviflora, M. fragrans and fluoxetine (the control drug for this study) increased serotonin, norepinephrine and dopamine in the rat hippocampus compared to that of the vehicle-treated group. Our proteomic data showed that 37 proteins in the K. parviflora group were up-regulated, while 14 were down-regulated, and 27 proteins in the M. fragrans group were up-regulated, while 16 were down-regulated. In the fluoxetine treatment group, we found 29 proteins up-regulated, whereas 14 proteins were down-regulated. In line with the proteomic data, the levels of GFAP, PDIA3, DPYSL2 and p-DPYSL2 were modified in the SD rat groups treated with K. parviflora, M. fragrans and fluoxetine as confirmed by Western blot. K. parviflora and M. fragrans mediated not only the levels of monoamine neurotransmitters but also the proteomic profiles in the rat hippocampus, thus shedding light on the mechanisms targeting neurodegenerative diseases.
參考文獻
延伸閱讀
- Kuo, Y. S. (2019). Concomitant Top-down Innervations from the Prefrontal Cortex onto Synaptically Connecting Interneurons and Norepinephrine Neuronsin Locus Coeruleus in Mice [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201903375
- 陳怡安（2004）。Part I: The effect of prenatal exposure to morphine on the expression of PSD-95 and synaptophysin in the hippocampus of developing rats. Part II: The therapeutic effect of serotonin reuptake inhibitor on the expression of morphine withdraw syndrome in ne〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714562754
- David, L. K., Fawehinmi, H. B., Ihunwo, A. O., & Worgu, E. (2018). Glial Fibrillary Acidic Protein Expression in the Hippocampal Formation of Mefloqine Induced-Seizured Rats Treated with Aqueous Leaf Extract of Luffa aegyptiaca Mill. Asian Journal of Medical Sciences, 9(1), 1-5. https://doi.org/10.19026/ajms.9.5828
- Yang, S., Yang, Y., Wan, F., Huang, C., & Liu, Y. (2017). Effects of Isolation Rearing and Early Antipsychotic Intervention on Oxidative Stress‑induced Apoptosis and Brain‑derived Neurotrophic Factor in Hippocampus in a Rat Model of Schizophrenia. Journal of Medical Sciences, 37(4), 155-162. https://doi.org/10.4103/jmedsci.jmedsci_113_16
- Chen, G., Shi, J., Ding, Y., Yin, H., & Hang, C. (2007). Progesterone Prevents Traumatic Brain Injury-Induced Intestinal Nuclear Factor kappa B Activation and Proinflammatory Cytokines Expression in Male Rats. Mediators of Inflammation, 2007(), 405-411. https://doi.org/10.1155/2007/93431