Gel formulations of Merremia mammosa (Lour.) accelerated wound healing of the wound in diabetic rats

Ancah Caesarina Novi Marchianti ； Elly Nurus Sakinah ； Ulfa Elfiah ； Nurin Kamila Suwandi Putri ； Deuxy Ilma Wahyuliswari ； Mizan Maulana ； Evi Umayah Ulfa

《Journal of Traditional and Complementary Medicine》 11卷1期 (2021/01) Pp. 38-45

https://doi.org/10.1016/j.jtcme.2019.12.002

摘要

Background and aim: The treatment of diabetic ulcers is difficult because of defective blood vessels and frequent co-occurrence of bacterial infections. In a previous study, we found a water fraction of Merremia mammosa (Lour.) (Mm(Lour.)) had beneficial effects on wound healing in diabetic rats. This study aimed to evaluate the influence of different gelling agents added to Mm(Lour.) water fraction gel on wound healing treatment in diabetic rats. Experimental procedure: Diabetic Wistar rats were divided into the following five groups: 1. positive control (Neomycin Sulfate 0.5% and Placenta Extract 10%), 2. negative control (distilled water), and 10% water fraction of Mm(Lour.) extract in 3. HPMC, 4. Carbopol, and 5. CMC Na gelling agents. The wound was made by the Morton method and treatment applied every other day for 25 days, then the wound healing process was observed. Data were observed and analysed using appropriate statistic tools. Results: Histopathology observation, VEGF expression and hydroxyproline levels showed a significant acceleration of wound healing in all treatment groups compared to the negative control group. This study showed all of Mm(Lour.) gel formulations could restore the delayed healing process on wound in diabetic rats and were equally effective in accelerating wound healing. CMC Na was the most preferable because it did not irritate. Conclusion: The results suggest that Mm(Lour.) water fraction in CMC Na gelling agent provided an option to be developed as a topical drug on diabetic wound healing treatment, showed by enhancement of collagen synthesis and angiogenesis.

關鍵字

Topical drug ； Gelling agent ； Diabetic wound

參考文獻

Heidari M, Bahramsoltani R, Hossein A. Efficacy of topical application of standardized extract of Tragopogon graminifolius in the healing process of experimental burn wounds. J Tradit Chinese Med Sci. 2019;9(1):54-59. https://doi.org/10.1016/j.jtcme.2018.02.002

Umeh VN, Ilodigwe EE, Ajaghaku DL, Erhirhie EO, Moke GE, Akah PA. Wound -healing activity of the aqueous leaf extract and fractions of Ficus exasperata (moraceae) and its safety evaluation on albino rats. J Tradit Complement Med. 2014;4(4):246-252. https://doi.org/10.4103/2225-4110.139105

Dwivedi D, Dwivedi M, Malviya S, Singh V. Journal of Traditional and Complementary Medicine Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Chinese Med Sci. 2017;7(1):79-85. https://doi.org/10.1016/j.jtcme.2015.12.002

Juneja K, Mishra R, Chauhan S, Gupta S, Roy P, Sircar D. Metabolite profiling and wound-healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models. J. Tradit., Complementary Altern. Med. 2019;xxxx. https://doi.org/10.1016/j.jtcme.2019.02.002

Singer AJ, Clark RAF. Cutaneous wound healing. Engl J Med. 1999;341:738-746. https://doi.org/10.1056/NEJM199909023411006