Dried mulberry fruit ameliorates cardiovascular and liver histopathological changes in high-fat diet-induced hyperlipidemic mice

Suriya Chaiwong ； Usana Chatturong ； Rachanee Chanasong ； Watcharakorn Deetud ； Kittiwoot To-on ； Supaporn Puntheeranurak ； Ekarin Chulikorn ； Tanwarat Kajsongkram ； Veerada Raksanoh ； Kroekkiat Chinda ； Nanteetip Limpeanchob ； Kanittaporn Trisat ； Julintorn Somran ； Nitra Nuengchamnong ； Piya Prajumwong ； Krongkarn Chootip

《Journal of Traditional and Complementary Medicine》 11卷4期 (2021/07) Pp. 356-368

https://doi.org/10.1016/j.jtcme.2021.02.006

摘要

Background and aim: Metabolic disease encompasses most contemporary non-communicable diseases, especially cardiovascular and fatty liver disease. Mulberry fruits of Morus alba L. are a favoured food and a traditional medicine. While they are anti-atherosclerotic and reduce hyperlipidemic risk factors, studies need wider scope that include ameliorating cardiovascular and liver pathologies if they are to become clinically effective treatments. Therefore, the present study sought to show that freshly dried mulberry fruits (dMF) might counteract the metabolic/cardiovascular pathologies in mice made hyperlipidemic by high-fat diet (HF). Experimental procedure: C57BL/6J mice were fed for 3 months with either: i) control diet, ii) HF, iii) HFt100 mg/kg dMF, or iv) HF+300 mg/kg dMF. Body weight gain, food intake, visceral fat accumulation, fasting blood glucose, plasma lipids, and aortic, heart, and liver histopathologies were evaluated. Adipocyte lipid accumulation, autophagy, and bile acid binding were also investigated. Results and conclusion: HF increased food intake, body weight, visceral fat, plasma total cholesterol (TC) and low-density lipoprotein (LDL), TC/HDL ratio, blood glucose, aortic collagen, arterial and cardiac wall thickness, and liver lipid. Both dMF doses prevented hyperphagia, body weight gain, and visceral fat accumulation, lowered blood glucose, plasma TG and unfavourable TC/HDL and elevated plasma HDL beyond baseline. Arterial and cardiac wall hypertrophy, aortic collagen fibre accumulation and liver lipid deposition ameliorated in dMF-fed mice. Clinical trials on dMF are worthwhile but outcomes should be holistic commensurate with the constellation of disease risks. Here, dMF should supplement the switch to nutrient-rich from current energy-dense diets that are progressively crippling national health systems.

關鍵字

Morus alba ； Mulberry fruit ； Hyperlipidemia ； Cardiovascular ； Liver

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