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Inhibitory effect of honokiol on furin-like activity and SARS-CoV-2 infection

摘要


The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a pandemic and has caused damage to the lives of the people and economy of countries. However, the therapeutic reagents against SARS-CoV-2 remain unclear. The spike (S) protein of SARS-CoV-2 contains a cleavage motif at the S1/S2 boundary, known to be cleaved by furin. As cleavage is essential for S protein activation and viral entry, furin was selected as the target compound. In this study, we examined the inhibitory effects of two lignans (honokiol and magnolol) on furin-like enzymatic activity using a fluorogenic substrate with whole-cell lysates. Of two compounds tested, honokiol partially inhibited furin-like enzymatic activity. We further examined the anti-SARS-CoV-2 activity of honokiol using VeroE6 cell line, which is stably expressing a transmembrane protease serine 2 (TMPRSS2). It was shown that honokiol exhibited remarkable inhibition of SARS-CoV-2 infection. Therefore, honokiol and crude drugs which contain honokiol such as Magnolia species have a potential therapeutic reagents for SARS-CoV-2.

關鍵字

SARS-CoV-2 Furin COVID-19 Honokiol Spike protein

參考文獻


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被引用紀錄


Isidoro, C., Chang, A. C. F., & Sheen, L. Y. (2022). Natural products as a source of novel drugs for treating SARS-CoV2 infection. Journal of Traditional and Complementary Medicine, 12(1), 1-5. https://doi.org/10.1016/j.jtcme.2022.02.001

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