Cyclone separator is mainly used for the separation of a small number of solid particles and droplets contained in the gas medium. The single-tube type cyclone separator is widely used in the oil and gas industry, food processing and biological science because of its simple structure, easy processing and high separation efficiency. Different applications and operating conditions on the separation performance of cyclone separator is not the same, through computational fluid analysis, USES the Fluent software the numerical simulation study on the internal flow field in cyclone separator, using two-way DPM model of fluid-solid coupling method for different size of particles in cyclone separator under different air inlet velocity of movement. The gas-solid separation efficiency was calculated by calculating the number of incident particles and escaping particles. The separation efficiency under a certain inlet velocity is in an exponential polynomial relationship with the particle size, and the separation efficiency under a certain inlet velocity of a certain particle size is in a logarithmic polynomial relationship within a certain range. When the inlet air velocity exceeds a certain value, the number of back mixing particles increases and the length of stay in the cyclone separator increases, resulting in an increase in the amount of erosion and wear in the separator.