In recent years, people's pursuit of the quality of production and life has gradually improved, and people have paid more attention to life health and environmental pollution. Gas chromatography/mass spectrometry technology can effectively determine complex organic matter qualitatively and quantitatively. This article reviews the application of gas chromatography/mass spectrometry technology in the determination of organic matter in soil and solid waste, water quality, food, biology, etc. in recent years, and briefly describes the current problems and application prospects of the technology.