Monomeric Proanthocyanidins Increase Intracellular NAD+Content by Enhancing NAMPT Transcription

Wenjing Wei ； Jinhua Zhang ； Yiting Fu ； Hansen Chen

《International Core Journal of Engineering》 8卷5期 (2022/05) Pp. 811-816

https://doi.org/10.6919/ICJE.202205_8(5).0104

摘要

Proanthocyanidins (PCs), a group of polyphenolic compounds that have been studied for almost 80 years, have a high antioxidant capacity and considerable benefits to human health. These compounds are effective at scavenging free radicals in organisms and have anti-inflammatory and antiaging effects. The content of nicotinamide adenine dinucleotide (NAD+) decreases with age. Exogenous supplementation with NAD+ plays a vital role in impeding aging and prolonging lifespan; thus, NAD+ is a potential target for antiaging research. There is evidence that PCs can affect the content of NAD+, but the effects of monomeric proanthocyanidins (MPCs) and oligomeric proanthocyanidins (OPCs) on NAD+ levels are still unclear. In our study, normal human diploid skin fibroblast BJ cells were treated with MPCs and OPCs, and changes in intracellular NAD+ content were examined. Real-time qPCR was used to detect changes in the transcription of enzymes associated with NAD+ synthesis. The results showed that both MPCs and OPCs could increase NAD+ content. In low-passage BJ cells, the effects of MPCs were greater than those of OPCs. Moreover, the qPCR results showed that the mRNA expression levels of the rate-limiting enzyme NAMPT were significantly upregulated in PC-treated low-passage and senescent BJ cells, suggesting that the effect of PCs on NAD+ content may be achieved by regulating NAMPT transcription, which may provide a theoretical basis for the effect of PCs on intracellular NAD+ content.

關鍵字

Proanthocyanidins ； NAMPT ； NAD+

參考文獻

She J, Sheng R, Qin Z-H J A, et al. Pharmacology and Potential Implications of Nicotinamide Adenine Dinucleotide Precursors[J], 2021, 12(8): 1879.

Hosseini L, Vafaee M S, Mahmoudi J, et al. Nicotinamide adenine dinucleotide emerges as a therapeutic target in aging and ischemic conditions[J], 2019, 20(4): 381-395.

Covarrubias A J, Perrone R, Grozio A, et al. NAD+ metabolism and its roles in cellular processes during ageing[J], 2021, 22(2): 119-141.

Pîrvu A S, Andrei A M, Stănciulescu E C, et al. NAD+ metabolism and retinal degeneration[J], 2021, 22(1): 1-6.

Ji L L, Yeo D J C. Maintenance of NAD+ Homeostasis in Skeletal Muscle during Aging and Exercise[J], 2022, 11(4): 710.