Low intensity pulsed ultrasound (LIPUS) is a therapeutic method that transmits mechanical energy into human tissues and cells in the form of ultrasonic resonance, and then produces a series of biological effects. It can regulate a series of signal transduction pathways in osteoblasts and osteoclasts. Some scholars have proposed that a series of biological effects produced by LIPUS are realized by regulating a series of non-coding Rnas (Ncrnas), especially micrornas. In this paper, the latest research progress and possible research direction of LIPUS on a series of biological effects of LIPUS on osteoblasts through non-coding RNA were discussed from the related mechanisms of LIPUS on osteoblasts and the related mechanisms of miRNA promoting bone healing.