By using the methods of literature, Mathematical Statistics and comparative analysis, this paper makes a comparative study on the effect of online and offline mixed teaching mode on the classroom teaching of sports anatomy. The results show that the students who use online-offline hybrid teaching mode have better performance than those who use traditional teaching mode. On-line and off-line mixed teaching mode can promote the change of students'learning attitude and improve their enthusiasm in learning sports anatomy. It is worth popularizing and applying in the teaching of other courses in the future.