- 會議論文
- OpenAccess
Identification of Imipenem-nonsusceptible Acinetobacter nosocomialis Isolates by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
摘要
Acinetobacter nosocomialis (A. nosocomialis) is one of the major pathogenic bacteria producing nosocomial infections. The presence of A. nosocomialis in hospital is detrimental to patients and to the hospital management. Rapid identification of imipenem-nonsusceptible A. nosocomialis can provide instant information for administration of antibiotics. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently become a widely used tool for identifying bacterial species. In this study, we constructed a classification model for identifying imipenem-nonsusceptible A. nosocomialis based on based on a large-scale MALDI-TOF MS dataset consisting of 1085 imipenem-nonsusceptible A. nosocomialis and 1047 imipenem-susceptible A. nosocomialis clinical isolates. The mass-to-charge ratio (m/z) values were mainly adopted to build the machine learning (ML) models, including random forest(RF), decision tree, and logistic regression. Finally, the performance of RF were the best among the other models. As a result, the RF-based prediction model for identifying imipenem-nonsusceptible A. nosocomialis can aid clinicians in administering the appropriate antibiotics.
延伸閱讀
- 葉婷安、歐宇祥、陳韋靜、蔡文城（2016）。Evaluation of the Suitability of Implementing Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Identification of Non-tuberculous Mycobacteria Commonly Encountered in Clinical Laboratories。檢驗及品保雜誌，5(4)，132-138。https://doi.org/10.6573/JTQA.201612_5(4).02
- 黃品瑜、陳羿樺、蔡文城（2017）。Evaluation of the Suitability of Implementing Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Identification of Legionellaceae Commonly Encountered in Aquatic Environment。檢驗及品保雜誌，6(1)，51-62。https://doi.org/10.6573/JTQA.201703_6(1).07
- Richel, A., Vanderghem, C., Simon, M., Wathelet, B., & Paquot, M. (2012). Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Second-Generation Lignin Analysis. Analytical Chemistry Insights, (), 79-89. https://doi.org/10.4137/ACI.S10799
- 李岱芬（2017）。Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of viridans group streptococci〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201700573
- Ou, Y. M. (2020). The Study of Effective Approaches for Carbohydrate Analysis in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU202000526