Proliferative Effects of Hyaluronic Acid on Human Dental Pulp Stem Cells

Shiau-Ting Shiu ； Sheng-Wei Feng ； Haw-Ming Huang

《Journal of Prosthodontics and Implantology》 7卷2期 (2018/07) Pp. 14-17

https://doi.org/10.6926/JPI.201807_7(2).0002

摘要

Purpose: Stem cells have become increasingly important in the development of tissue engineering. Among them, dental pulp stem cells can be obtained in a non-invasive manner. In recent years, advances in technology have enabled dental pulp stem cells to be successfully applied to periodontal diseases and the regeneration of the lower jaw bone, in addition to creating the potential for more applications due to the self-regeneration and multi-differentiation capabilities of stem cells. Hyaluronic acid (HA) is a natural biological polymer that has important physiological functions in vertebrates and even bacteria. Past studies have indicated that HA, in addition to being an important material in tissue engineering, is related to the physiological activities of specific cells or even stem cells. Therefore, this study sought to explore the effect of HA on dental pulp stem cells. Material and methods: Dental pulp stem cells were cultured with 20 KGy or 60 KGy γ-ray treated HA. Their cell viability was then tested by MTT assay. Results: Our results showed that there was no significant difference between the control group and experimental groups. Conclusion: That is, the results suggested that HA does not exhibit the ability to improve the viability of dental pulp stem cells. However, further experimental studies with various HA concentrations are still needed to determine whether HA has any positive effects on dental pulp stem cells.

關鍵字

dental pulp stem cell ； hyaluronic acid ； tissue engineering ； x-ray