From 2011, the Chinese mainland carried out a new round of research on Chinese National Sign Language, and published a common sign language vocabulary and a national sign language dictionary. Based on the Chinese National Sign Language, this paper discusses the relationship between morphemes and word order in sign language from three aspects: 1) the relationship between verbs and word order, 2) the relationship between the nature of morphemes and word order in sign language, 3) and the form of antisense expression in sign language.