盤固草化學成分近紅外光分析檢量線之建立

本研究目的為建立盤固草品質近紅外光分析檢量線，並以獨立樣品進行測試。測定成分包括：粗蛋白質、酸洗纖維、中洗纖維及水溶性碳水化合物。以三批不同來源樣品計294個，混合用於建立基礎檢量線。粗蛋白質、酸洗纖維、中洗纖維及水溶性碳水化合物基礎檢量線之決定係數（R^2）分別為0.98、0.85、0.81及0.93，檢量線標準機差（SEC）分別為0.49%、1.78%、2.20%及0.97%，交互驗證標準機差（SECV）分別為0.57%、1.89%、2.28%與1.22%。基礎檢量線建立後之次年，由數地區酪農場收集盤固草樣品計125個進行測試與更新。測試結果，粗蛋白質、酸洗纖維、中洗纖維及水溶性碳水化合物之平均值偏差分別為0.0、0.77、0.27及0.16，表示基礎檢量線對不同來源樣品已有相當高的準確度。其後將測試用樣品加入基礎資料庫，進行檢量線更新，新檢量線粗蛋白質、酸洗纖維、中洗纖維及水溶性碳水化合物之R^2分別為0.99、0.85、0.88及0.96，SEC分別為0.36%、1.73%、1.61%及0.81%，交互驗證標準機差分別為0.42%、1.81%、1.80%及0.92%，樣品實測值標準偏差與驗證標準機差之比值（RPD）分別為9.0、2.6、2.5及4.3，均達可接受之準確度。本結果提供盤固乾草的品質快速測定法，可做為健全國產乾草產銷的工具。水溶性碳水化合物含量的快速測定，亦有助於盤固草的青貯調製。

關鍵字

快速測定；粗蛋白質；中洗纖維；酸洗纖維；水溶性碳水化合物；牧草品質

並列摘要

Objective of this study was to establish the calibration equations for determining the chemical components of pangolagrass using near infrared spectroscopy (NIRS). The equations were verified using independent sample sets. The determined constituents included crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and water-soluble carbohydrates (WSC). Two hundred ninety-four samples from three sources were used to set up the basic calibration. The R^2 of the regressions for CP, NDF, ADF, and WSC were 0.98, 0.85, 0.81, and 0.93, SEC (standard error of calibration) were 0.49%, 1.78%, 2.2%, and 0.97%, and SECV (standard error of cross verification) were 0.57%, 1.89%, 2.28%, and 1.22%, respectively. The basic calibration equations were verified with another 125 samples collected from dairy farms from different areas.. The results showed that the biases for the CP, NDF, ADF, and WSC means were 0, 0.77, 0.27, and 0.16, respectively. This indicated that the basic calibration equations were accurate for samples from different sources. The new sample sets were added to update the basic calibration. The R^2 of the updated regressions for CP, NDF, ADF, and WSC were 0.99, 0.85, 0.88, and 0.96, SEC were 0.36%, 1.73%, 1.61%, and 0.81%, and SECV were 0.42%, 1.81%, 1.80%, and 0.92%, respectively. The RPD(the ratio of the standard deviation of constituent to the standard error of prediction)were 9.0, 2.6, 2.5, and 43 for CP, NDF, ADF, and WSC, respectively. All of the calibration equations were acceptable for accurately predicting the above-mentioned constituents. The hay quality of pangolagrass was quite variable. The results might be helpful to provide a rapid method for quality analysis of pangolagrass hay and for use as a tool to facilitate the hay trade in the courtry. Further, the rapid analysis of WSC would be also helpful for pangolagrass to make silage.

並列關鍵字

Rapid analysis ； Crude protein ； Acid detergent fiber ； Neutral detergent fiber ； Water-soluble carbohydrates ； Forage quality

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