豬糞尿厭氣處理污泥中之甲烷菌分布探討

本研究目的在探討不同豬糞尿廢水處理系統厭氣污泥中之甲烷菌分布，及其對有機物處理效率之影響。因此，利用16S rDNA聚合瓷鏈反應、限制瓷圖譜、序列分析及親緣分析法進行三段式豬糞尿廢水處理系統厭氣污泥（T）與北卡州立大學農場上流式厭氣處理模場污泥（A）中甲烷菌分布探討。結果在厭氣處理污泥T和A中，分別得到63和51個株系，並依限制瓷分析結果，分為13和15個操作型分類單位（OTU）。親緣分析結果顯示污泥T中，50.8%甲烷菌屬於Methanosarcinales目，42.9%屬於Methanomicrobiales目，另外6.3%則未能歸於已知菌目。然而，污泥A中未有Methanosarcinales與Methanomicrobiales目，僅9.8%屬於Methanobacteriales目，29.4% Methanococcales目，60.8%未能歸於已知菌目。兩種污泥之甲烷菌分布顯然差異很大，而嗜乙酸甲烷菌Methanosaeta concilii佔多數，應是三段式系統有機物去除率高之原因。

關鍵字

厭氣污泥；甲烷菌；操作型分類單位

並列摘要

The aim of this study was to examine the methanogenic population structures of different anaerobic bioreactors treating swine wastewater, to evaluate their influence on the efficiency of bioreactors. This was accomplished by 16S rDNA clone library construction, amplified rDNA restriction analysis (ARDRA), DNA sequence analysis and phylogenetic analysis of archaeal gene sequences, which were amplified from the anaerobic sludges sampled from the three-step swine wastewater treatment system(T) and UASB pilot plant(A). Of 63 and 51 clones obtained from sludge T and A, respectively, 13 and 15 OTUs were defined by ARDRA. And, the results of phylogenetic analysis showed 50.8% of methanogenic clones were classified as order Methanosarcinales, 42.9% of those as order Methanomicrobiales, and the other 6.3% as unkown archaea in sludge T. However, there was no clones classified as order Methanosarcinales or Methanomicrobiales, while 8% of those were classified as order Methanobacteriales, 29.4% as order Methanococcales, and 60.8% as unkown archaea in sludge A. It was clear that the methanogenic population structures between these two kinds of sludges were different. And the result suggested that the domination of acetotrophic Methanosaeta concilii in sludge T might be the reason for high organic removal efficiency of three-step swine wastewater treatment system.

並列關鍵字

Anaerobic sludge ； Methanogen ； Operational taxonomy unit (OTU)

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