豬冷凍精液解凍溫度及時間之探討

Effects of thawing temperature and time on boar frozen semen

章嘉潔(Chia-Chieh Chang)

《畜產研究》 42卷4期 (2009/12) Pp. 361-369

摘要

本試驗探討四種解凍條件（50℃,50秒、45℃,50秒、50℃,45秒及45℃,45秒）對豬冷凍精液解凍後品質的影響。選擇生殖能力正常、平均年齡2.5歲之健壯杜洛克公豬8頭，採集其精液製作冷凍精液，並比較解凍條件對解凍後之精子存活率、精子總活力、精子快速前進式活力等項目之差異。試驗結果顯示，解凍條件分別為50℃,50秒及50℃,45秒，對於解凍後精子之存活率、精子之總活力、精子之快速前進式活力等，在統計上均無顯著差異；而解凍條件為50℃,45秒，則對於解凍後之精子存活率、精子總活力、精子快速前進式活力等均顯著優於解凍條件為45℃,45秒組（P＜0.05）。

關鍵字

精液 ； 解凍 ； 豬

並列摘要

This experiment was conducted to investigate the effects of thawing conditions at 50℃, 50 sec, 45℃, 50 sec、50℃, 45 sec and 45℃, 45 sec respectively on quality of frozen- thawed semen in boars. Semen collected from eight healthy Duroc boars of known reproductive history (2.5 year of age), were retained for sperm cryopreservation. Frozen semen with different thawing conditions and the percentage of live sperm, total motility and rapid progressive motility (RPM) were investigated. The results showed that the percentages of live sperm, total motility and RPM were not significantly different between the two groups of thawed at 50℃, 50 sec and 50℃, 45 sec. (P ＞ 0.05). Thawing straw at 50℃, 45 sec had significantly (P ＜ 0.05) higher percentage of live sperm, total motility and RPM than those of straws thawed at 45℃, 45 sec.

並列關鍵字

Semen ； Thawing ； Boar