體細胞核轉殖乳山羊的端粒長度分析

Telomere length analysis of cloned dairy goat produced by somatic cell nuclear transfer

蕭振文(Jen-Wen Shiau) ； 蔡麗卿(Lee-Ching Tsai) ； 王君今(Chun-Chin Wang) ； 陳裕信(Yu-Hsin Chen) ； 曲鳳翔(Feng-Hsiang Chu) ； 劉振發(Jenn-Fa Liou) ； 黃政齊(Jan-Chi Huang) ； 林正鏞(Cheng- Yong Lin) ； 戴謙(Chein Tai) ； 陳立人(Lih-Ren Chen)

《畜產研究》 43卷1期 (2010/03) Pp. 31-40

摘要

本研究主要目的在分析比較畜產試驗所應用體細胞核轉置（somatic cell nuclear transfer, SCNT）技術生產的3頭阿爾拜因複製山羊與同年齡同性別非複製乳山羊的端粒（telomere）長度，藉以了解SCNT對於阿爾拜因複製山羊之端粒長度的影響。試驗分別自3頭複製羊於不同月齡時採集全血經離心分離並收集白血球供萃取基因組DNA，再應用套組完成端粒長度（terminal restriction fragment, TRF）分析。結果顯示，3頭複製羊於不同月齡之端粒長度均顯著地（P ＜0.05）比同月齡同性別之非複製對照山羊為短。

關鍵字

體細胞核轉置 ； 端粒長度 ； 山羊

並列摘要

The purpose of this study was to determine the telomere length of somatic cell nuclear transfer (SCNT) cloned dairy goat and their age-match control counterparts. Genomic DNA samples were purified from whole blood of cloned and control animals and telomere length were assessed by terminal restriction fragment (TRF) analysis. The results showed that the telomere length were significantly (P ＜ 0.05) shorter in three SCNT dairy goats cloned from cultured adult somatic cells as compared to age-matched control goats.

並列關鍵字

Somatic cell nuclear transfer (SCNT) ； Telomere length ； Goat