不同稀釋液及去除精漿對山羊精液冷藏保存之影響

Effects of different extenders and removal of seminal plasma on goat semen quality during storage at 4℃

吳昇陽(Sheng-Yang Wu) ； 沈朋志(Perng-Chih Shen) ； 章嘉潔(Chia-Chieh Chang)

《畜產研究》 47卷3期 (2014/09) Pp. 153-160

摘要

本研究探討稀釋液及去除精漿對山羊精液冷藏後精液品質之影響。精液採集後區分為有無去除精漿等兩種處理；即分別為未去除精漿且添加脫脂乳粉（SKM-NW）或蛋黃稀釋液（YTF-NW），以及去除精漿且分別添加脫脂乳粉（SKM-W）或蛋黃稀釋液（YTF-W）等處理組。試驗一、將稀釋精液冷藏於4℃後，回溫37℃培養0、2、4及6小時，精液性狀評估結果顯示，未去除精漿之精液添加脫脂乳（SKM-NW）或蛋黃（YTF-NW）之稀釋液，對精子活力、前進式活力及頭帽完整性皆無顯著差異，而去除精漿之精液添加脫脂乳（SKM-W）或蛋黃（YTF-W）之稀釋液於冷藏4℃後，回溫37℃培養4及6小時，在精子活力、前進式活力及頭帽完整性則呈顯著差異（P＜0.05）。試驗二、去除精漿對山羊精液冷藏3日後之精子活力、前進式活力及精子頭帽完整性之影響，結果顯示冷藏在4℃下經0、1、2及3日，各處理組之精子活力、前進式活力及頭帽完整性均無顯著差異，而冷藏於4℃下3日，去除精漿之精液添加脫脂乳（SKM-W）或蛋黃（YTF-W）之稀釋液，在精子活力、前進式活力及頭帽完整性皆呈顯著差異。去除精漿之精液添加脫脂乳粉（SKM-W）冷藏者與其他組比較，對精子之保護力較差。結論顯示，精液去除精漿並添加以脫脂乳粉為基礎成分之稀釋液（即SKM-W處理組），較不適用於山羊精液之冷藏保存。

關鍵字

山羊精液 ； 稀釋液 ； 冷藏保存

並列摘要

The purposes of this study were to evaluate the effects of extender and centrifugation and removal of seminal plasma on semen quality. The ejaculates were pooled and divided into two aliquots, the semen plasma of one aliquot was remained intact and diluted with for skimmed-milk based (SKM-NW) or egg yolk based extender (YTF-NW) and for the other aliquot the semen plasma was removed and then diluted with skimmed-milk based (SKM-W) or egg yolk based extender (YTF-W), respectively. In experiment 1, results showed that there were no differences in sperm motility, progressively motility and acrosome integrity among the SKM-NW and YTF-NW stored at 4℃ and then incubated at 37℃ after 0, 2, 4 and 6 h. However, there were significant (p ＜ 0.05) difference on the sperm motility, progressively motility and acrosome integrity between the SKM-W and YTF-W stored at 4℃ and then incubated at 37℃ for 4 and 6 h (P ＜ 0.05). In experiment 2, results showed that there were no differences in sperm motility, progressive motility and acrosome integrity between the SKM-NW and YTF-NW stored during 0, 1, 2 and 3 day at 4℃. However, the SKM-W treatment had lower (P ＜ 0.05) sperm motility, progressive motility and acrosome integrity than that of the YTF-W during storage at 4℃ for 3 days. In conclusion, SKM-W was not suitable for the storage of goat semen at 4℃.

並列關鍵字

Goat semen ； Extenders ； Refrigeration