臺灣乳牛群短脊椎綜合症基因檢測之初探

The pilot study on genotype screening of brachyspina syndrome on dairy cattle herds in Taiwan

廖仁寶(Ren-Bao Liaw) ； 陳若菁(Jo-Ching Chen) ； 陳玟岑(Wen-Cen Chen) ； 蔡新興(Shin-Shing Tsay) ； 黃金山(Chin-Shan Huang) ； 陳志毅(Jih-Yih Chen) ； 蕭宗法(Tzong-Faa Shiao) ； 李國華(Kuo-Hua Lee) ； 周宜昌(Yi-Chang Chou) ； 吳明哲(Ming-Che Wu) ； 張秀鑾(Hsiu-Lan Chang)

《畜產研究》 48卷3期 (2015/09) Pp. 210-215

摘要

乳牛短脊椎綜合症為一隱性遺傳缺陷，會導致高乳量牛群之懷孕母牛流產或死產之重大經濟損失。本研究旨在建立與檢測乳牛族群短脊椎綜合症基因型，藉以瞭解臺灣乳牛群中該基因型頻率。試驗樣品總計有441個，分別為來自四家乳牛場之409頭乳牛血液樣品與32個乳牛冷凍精液樣品。試驗結果顯示，在所有檢測的乳牛樣本中，其基因型鑑定結果，35個乳牛血液樣品與1個冷凍精液樣品為雜合型，雜合型頻率分別為8.56與3.13%，其餘樣品皆為正常型。檢測結果顯示我國乳牛群中，雜合型頻率不可忽視，故如何減少酪農產業之潛在損失，應為當務之急。因此，有必要嚴密監控冷凍精液之短脊椎綜合症基因型與篩檢仔牛群，防止短脊椎綜合症雜合型個體持續進入我國乳牛族群，俾利逐步篩除此一不良基因。

關鍵字

短脊椎綜合症 ； 乳牛 ； 基因型鑑定 ； 冷凍精液

並列摘要

Brachyspina syndrome (BS) is inherited autosomal recessively in Holstein cattle, which would result in miscarriage or stillbirth for dairy cows and thus cause unpredictable economic losses to dairy farmers. The purpose of this study is to reveal bovine brachyspina syndrome carriers frequency in dairy cattle population. A total of 441 samples were genotyped, which were 409 blood samples from four dairy farms and 32 frozen bovine semen samples. Thirty-five blood samples and one frozen semen were identified as BS carriers and the others were normal. The frequencies of carriers for blood samples and frozen semen were 8.56 and 3.13%, respectively. Because of high frequency in BS carrier genotype, it implies that the dairy farmers have potential losses due to BS. Therefore, the BS genotypes of bovine frozen semen needs to be under surveillance and ensure no BS carrier calves into dairy cattle population in Taiwan. Thus, it is possible to elimate gradually the genetic deficiency in the future.

並列關鍵字

Brachyspina syndrome ； Dairy cattle ； Genotyping ； Frozen semen