雞單一性別始基生殖細胞移殖之胚胎存活率探討

本研究之目的係探討單一性別之家禽始基生殖細胞（primordial germ cell, PGC）進行活體移植後之胚胎存活率與性腺遷徙效率。供試PGC是分離自孵化5.5天的早期雞胚胎之性腺，PGC移植後之胚胎存活率與性腺遷徙效率之評估，則是將轉染綠色螢光蛋白（green fluorescent protein, GFP）報導基因的PGC，注入（2μL，150－300 cells/μL）到孵育3.5天（stage 12－15）胚胎的背大動脈，進行活體移植測試。結果顯示，經PGC移植後孵育至第14天之胚胎存活率，以來亨雞的PGC移植到畜試土雞的胚胎存活率顯著高於以畜試土雞的PGC移植到來亨雞的胚胎者（50.7% vs. 17.4%；P＜0.05）；胚胎性腺GFP報導基因的檢測呈現陽性反應者，在來亨雞PGC移植到畜試土雞胚胎者為17.4%（18/103），而畜試土雞PGC移植到來亨雞胚胎者為15.5%（5/33）。本研究之結果將可供作後續家禽基因轉殖研究之參考。

關鍵字

雞；始基生殖細胞；單一性別；活體移植；胚胎存活率

並列摘要

The objectives of this study were to evaluate embryo survival and gonadal migration rate of sex-limited PGCs after transplantation. Chicken PGCs collected from the primitive gonads of chicken embryos (5.5-day-old) were used as donor cells for transplantation into the recipient chicken embryos. Before transplantation, an EGFP reporter gene was transferred into the PGCs. Approximately 2 μL of electroporated PGC suspension containing 150-300 cells/μL were injected into the dorsal aorta of the recipient chicken embryos of 3.5-day-old. The results showed that the survival rate at day 14 after incubation in embryos of TLRI native chicken transplanted with Leghorn PGCs was higher than the Leghorn embryos transplanted with TLRI PGCs (50.7% vs. 17.4%; P ＜ 0.05). The expression rates of GFP gene detected in the gonads of transplanted embryos was 17.4% (18/103) and 15.5% (5/33) in TLRI native chicken and Leghorn chicken embryos examined at 14 days of incubation, respectively. Results of this study can facilitate further transgenic chicken studies in the future.

並列關鍵字

Chicken ； Primordial germ cell ； Sex-limited ； In vivo transplantation ； Embryos survival rate

國際替代計量

雞單一性別始基生殖細胞移殖之胚胎存活率探討

全文下載

主題瀏覽