精子濃度與個體因素對豬精子冷凍解凍後存活率之影響

The effects of semen concentration and individual boars on the frozen-thaw sperm viability

王錦盟(Chin-Meng Wang) ； 陳裕信(Yu-Hsin Chen) ； 張雁智(Yen-Chih Chang) ； 粘碧珠(Pi-Chu Nien) ； 胡見龍(Chien-Lung Hu) ； 陳立人(Li-Ren Chen)

《畜產研究》 49卷2期 (2016/06) Pp. 157-161

摘要

本試驗之目的在探討豬冷凍精液濃度與公豬個體因素對解凍後精子存活率的影響。以彰化種畜繁殖場之公豬為試驗動物，進行豬冷凍精液製備，解凍後進行人工授精試驗，其受胎率與產仔頭數分別為67%（8／12）與9.4 ± 2.4頭／胎。另於A與B民間場進行人工授精試驗，其受胎率／產仔頭數分別為66.7%（8／12）／9.2 ± 2.4頭／胎與57.1%（4／7）／12.0±2.2頭／胎，得到類似的結果。顯示以本試驗製程製作的豬冷凍精液，解凍後具有受精能力，可使母豬受胎並產出後裔。另採集6頭杜洛克公豬精液經稀釋後以等濃度等比率混合，再調整濃度使製成豬冷凍精子最終濃度分別為13.1、10.0、7.8、6.4與5.6 × 10^8精子／mL。經解凍後濃度6.4 × 10^8精子／mL的精子存活率最高為43.3 ± 9.0%。另採集7頭藍瑞斯與17頭杜洛克精液為試驗材料，分別製成高精子濃度10.0 × 10^8與低精子濃度6.4 × 10^8精子／mL的冷凍精液，解凍後低精子濃度的存活率為36.3 ± 2.9%，比高精子濃度的25.8 ± 2.7%為佳。又活精子比率30%以下的頭數比值，在高與低精子濃度分別為10／24（41.7%）與6／24（25.0%）。結果顯示在此冷凍精液製作條件下，高濃度之豬冷凍精液解凍後的精子存活率較低，而個體因素造成解凍後豬精子存活率偏低的公豬比率為25%。

關鍵字

公豬 ； 精液 ； 冷凍 ； 受胎率 ； 產仔頭數

並列摘要

The purposes of this study were to evaluate the effect of both of boar and frozen boar semen concentration on the frozen-thaw sperm viability. The freeze boar semen were produced using boars from Changhua Animal Propagation Station and than thaw and insemination. The conception rate and litter size were 67% (8/12) and 9.4 ± 2.4 fetus/pig. The similar results were gotten from 2 farms. Another way the 6 Duroc semen samples were mixed with same concentration and then frozen at 13.1, 10.0, 7.8, 6.4 and 5.6 × 10^8 sperms/mL concentration. After frozen-thaw, the viability of sperm using fluorescent method shows that the 6.4 × 10^8 sperms/mL concentration frozen-thaw semen had 43.3 ± 9.0% live sperm, significant higher than the 13.3×10^8 sperms/mL concentration, 14.5 ± 2.9% (P ＜ 0.05). On the other study, the 24 semen samples were collected from 7 Landrace and 17 Duroc boars. High and low frozen semen concentrations, 10.0 and 6.4 × 10^8 sperms/mL, were made at each sample. The viability of sperm in the frozen-thaw semen was determined by microscopy method, the result shows that the low concentration samples had 36.3% motile sperm higher than the high concentration 25.8%. The number and ratio of the boars having lower than 30% motile sperm were calculated and the ratios of boar at high and low concentration were 41.7% (10/24) and 25.0% (6/24). However, it seems that using our program to make frozen boar semen, the frozen-thaw semen at high concentration had lower live sperm ratio. The boar effect bring out lower ratio live sperm in frozen-thaw semen was not less than 25%.

並列關鍵字

Boar ； Semen ； Cryopreservation ； Conception rate ； Litter size