- 期刊
外源激性腺素對荷蘭乳牛活體取卵之影響
Effects of exogenous gonadotropin on ovum picked-up of Holstein cows
摘要
本試驗目的在探討荷蘭乳牛活體取卵時，以外源激濾泡素（follicle-stimulating hormone, FSH）處理對於抽取濾泡數、回收卵母細胞數及培養後胚發育之影響。試驗以8頭乾乳牛逢機分成2組，連續進行8週，每週活體取卵一次，由全程抽取濾泡數、收集卵母細胞數及發育至囊胚率，以評估處理的差異。在牛隻沒有施打激濾泡素的組別中，計抽取濾泡數101個及回收卵母細胞26個，平均每頭每次抽取濾泡數為3.1 ± 1.3；回收卵母細胞數為0.8 ± 0.6。經培養後其卵裂率為46.15%（12/26），發育至囊胚率為0%（0/12）。相較於牛隻經施打激濾泡素的組別中，於抽除優勢濾泡之後的36小時，注射激濾泡素20A.U.（8A.U., I.M. 及12A.U., S.C.），並於注射激濾泡素後48小時進行活體取卵（ovum pick-up, OPU），計抽取濾泡數284個及回收卵母細胞數87個，平均每頭每次抽取濾泡數為8.6 ± 3.6；回收卵母細胞數為2.7 ± 1.8。經培養後其卵裂率為47.13%（41/87），發育至囊胚率為5/41（12.20%）。上述試驗結果顯示，牛隻經外源性激濾泡素誘發濾泡成熟，每週進行活體取卵一次，無論是抽取濾泡數、卵母細胞回收數、培養後之卵裂率與發育至囊胚率，均顯著優於未經外源性激濾泡素處理者（P ＜ 0.05）。
並列摘要
Ovum pick-up (OPU) made available oocytes from living cows, presenting a novel opportunity to use germplasm of known origin. Subsequently, it became increasingly apparent that the combination of OPU and in vitro production (IVP) techniques could be a potential alternative to traditional embryo production. Two groups of dry cows (4 heads × 8 weeks in each group) were conducted to evaluate the numbers of follicle and oocyte collected and the development of blastocyst. In non-stimulated group, 101 follicles were aspirated and 26 oocytes were retrieved, in other words, 3.1 ± 1.3 follicles and 0.8 ± 0.6 oocytes were obtained per head per operation. After culturing, the cleavage rate was 46.15% (12/26) but blastocyst development rate was 0% (0/12). In hormone-stimulated group which received FSH (8 A.U., I.M. plus 12 A.U., S.C.) 36 hrs after previous dominant follicle aspiration then carried out OPU operation 48 hrs after FSH injection. There were 284 follicles aspirated and 87 oocytes retrieved, i.e. 8.6 ± 3.6 follicles and 2.7 ± 1.8 oocytes per head per operation. Cleavage rate was 47.13% (41/87) and blastocyst development rate was 12.20% (5/41). The results showed that there were significant increase on follicle aspiration, oocyte retrieval, cleavage rate and blastocyst development rate in FSH-stimulated group on the basis of one week interval (P ＜ 0.05).
延伸閱讀
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- 邱雅芳（2019）。荷蘭牛來源輸卵管幹細胞之鑑定與分化及其對乳房炎與黃體囊腫治療之探討〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST201900110
- 溫婧暄（2020）。荷蘭牛擠乳效能與產乳性能相關標記基因探討〔碩士論文，國立屏東科技大學〕。華藝線上圖書館。https://doi.org/10.6346/NPUST202000266
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