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血統登錄種豬基因多樣性分析
Evaluating genetic diversity of pedigreed and registered breeding pigs by microsatellite markers
摘要
畜養動物基因多樣性維護，為生物多樣性公約愛知目標與聯合國永續發展目標重要項目之一。本研究應用29組豬微衛星遺傳標記，分析64頭杜洛克、63頭藍瑞斯及62頭約克夏品種之種豬DNA樣品，計189個。經分析後得知三個品種種豬平均交替基因數（Na）、有效交替基因數（Ne）、觀測異質度（Ho）、期望異質度（He）、多態性訊息量（PIC）、族群近交係數（F_(IS)）及族群分化指數（F_(ST)）分別為9.9、4.2、0.615、0.718、0.682、0.0453及0.1459。三個品種種豬之個別平均PIC值均大於0.5，尤以約克夏豬種PIC值最大，顯示種豬族群具有高度遺傳多態性資訊。此外，族群結構分析發現，所有樣品最適分成三群，各群最主要由單一品種組成，且每一分群的主要品種組成頻率皆達95%以上，推測我國種豬族群已相當純化。
並列摘要
The genetic diversity protection of domesticated animals is one of the important targets of Aichi Biodiversity Targets of the Convention on Biological Diversity and the Sustainable Development Goals (SDGs) of the United Nations. A total of 189 breeding pigs including 64 Duroc, 63 Landrace, and 62 Yorkshire pigs were genotyped using 29 microsatellite markers. In this study, the values of mean number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), F_(IS) and F_(ST) were 9.9, 4.2, 0.615, 0.718, 0.682, 0.0453, and 0.1459 for the three breeds of breeding pigs. Moreover, the average PIC values within breed for three breeds evaluated were larger than 0.5. Particularly, the PIC of Yorkshire pigs was the largest among the three breeds of pigs. The results indicated that the pig populations possess high genetic diversity. Besides, the clustering results showed that all samples could be classified into appropriate three clusters and each cluster was composed of one major breed with more than 95% in frequency. It implies that the tested breeding pigs are quite pure in the specific breed.
延伸閱讀
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