不同消毒處理對褐色菜鴨種蛋微生物與孵化之影響

Effects of different disinfection treatments on microorganisms and hatchability of Brown Tsaiya ducks' eggs

鄭智翔(Chih-Hsiang Cheng) ； 蘇晉暉(Chin-Hui Su) ； 吳弘毅(Hung-Yi Wu) ； 劉秀洲(Hsiu-Chou Liu) ； 林榮新(Jung-Hsin Lin)

《畜產研究》 52卷1期 (2019/03) Pp. 27-36

摘要

本試驗旨在探討不同消毒處理對褐色菜鴨種蛋微生物與孵化之影響，試驗將收集之種蛋逢機分為7組，分別以3倍甲醛燻蒸30分鐘、以霧化之250 ppm二氧化氯處理30分鐘、以2.2%臭氧氣體處理30分鐘、以4.4%臭氧氣體處理30分鐘、以500 ppm電解氧化水、500 ppm四級銨化物及20 ppm奈米銀離子溶液噴霧處理20分鐘，測試對種蛋之蛋殼表面生菌數與孵化之影響，並進行不同消毒處理對孵化7天及28天中止鴨胚之病原分析。結果顯示，不同消毒處理均可顯著降低蛋殼表面之生菌數；各處理組之受精率、25－28天中止率、入孵蛋孵化率及受精蛋孵化率均無顯著差異，然而在0－25天中止率部分，以霧化之250 ppm二氧化氯30分鐘處理組最高。孵化7天中止鴨胚之病原分析結果顯示，以濃度2.2%及4.4%臭氧氣體30分鐘處理組，對多種微生物均有明顯清除效果，其次則為以霧化之250 ppm二氧化氯30分鐘處理組；從孵化28天中止鴨胚之病原分析來看，可發現孵化過程中，環境微生物有持續增長之現象。

關鍵字

種蛋 ； 褐色菜鴨 ； 消毒

並列摘要

The aim of this experiment was to investigate the effects of different disinfection treatments on the effect of microorganisms and hatchability of Brown Tsaiya ducks' eggs. In the experiment, the breeding eggs were divided into 7 groups, which were treated with 3 times of formaldehyde fumigation for 30 mins, treated with 250 ppm atomized chlorine dioxide for 30 mins, 2.2% and 4.4% ozone gas for 30 mins, spray with 500 ppm electrolytic oxidation water, 500 ppm quaternary ammonium compound and 20 ppm nano-silver solution for 20 mins, respectively. The disinfection effect on the number of microorganisms on the eggshell surface and hatching of the breeding eggs were determined. In addition, the pathogens in dead embryos 7 and 28 days after incubation were identified. The results showed that different disinfection methods could significantly reduce the number of the microorganisms on the eggshell surface. In terms of the effect on hatching the breeding eggs, there were no significant differences in fertilization rate, the discontinuation rate of 25-28 days of incubation, hatching rate of incubation eggs and fertilized eggs. However, the group treated with 250 ppm atomized chlorine dioxide for 30 mins had the highest discontinuation rate at 25 days of incubation. The pathogen analysis of the dead embryo at 7 days of incubation showed that the group treated with 2.2% and 4.4% ozone gas for 30 mins had significant effect on reducing various microorganisms, followed by the group treated with atomized 250 ppm chlorine dioxide for 30 mins. The results of the pathogen analysis of the dead embryo at 28 days of incubation showed that the environmental microorganisms continued to grow during the incubation period.

並列關鍵字

Breeding Egg ； Brown Tsaiya duck ； Disinfection