山羊腹腔鏡卵母細胞採集技術之建立

Establishment of goat laparoscopic oocytes pick-up technology

康定傑(Ting-Chieh Kang) ； 曾楷扉(Kai-Fei Tseng) ； 林彥均(Yen-Chun Lin) ； 陳裕信(Yu-Hsin Chen) ； 曲鳳翔(Fung-Hsiang Chu) ； 林信宏(Hsin-Hung Lin) ； 沈朋志(Perng-Chih Shen)

《畜產研究》 54卷2期 (2021/06) Pp. 91-98

https://doi.org/10.6991/JTLR.202106_54(2).0003

摘要

本研究主要目的為建立山羊腹腔鏡卵母細胞採集技術，並與傳統外科手術取卵方式進行比較。試驗使用臺灣黑山羊恆春品系及努比亞羊隻各10頭。山羊卵母細胞於採集後，接續進行體外成熟、體外受精及體外培養以觀察卵母細胞成熟率、卵裂率、囊胚率及囊胚細胞數。體外成熟率之評估結果顯示，無論何種卵母細胞採集方法，卵母細胞經24小時體外成熟培養後，細胞核可成功發育並停滯在第二次減數分裂中期（Metaphase II, MII）者無顯著差異（74.88 ± 2.00% vs. 81.14 ± 7.00%）。體外受精培養24小時後之受精卵續進行48小時培養後可以觀察到卵裂。不同卵母細胞收集方法（外科手術vs.腹腔鏡法）卵裂率（73.93 ± 1.90% vs. 68.30 ± 3.99%）及受精後第7天之囊胚率（42.87 ± 1.26% vs. 42.65 ± 3.83%）均無顯著差異；但囊胚細胞數則以外科手術法顯著高於腹腔鏡法（76.41 ± 1.29 vs. 69.00 ± 8.91, P＜0.05）。本研究成功建立了腹腔鏡卵母細胞收集技術，收集到的卵母細胞經體外培養後之囊胚發育能力不遜於外科手術收集者，腹腔鏡卵母細胞採集技術確實可用於山羊卵母細胞收集。

關鍵字

山羊 ； 腹腔鏡卵母細胞收集 ； 囊胚率

並列摘要

The aims of this study were to establish the technique of laparoscopic oocytes pick-up and compare the techniques with the traditional surgical oocytes collection methods of goat. Twenty goats were used in this study (10 Hengchun line of Taiwan Black Goat and 10 Nubian goat), followed by conducting in vitro maturation, insemination, and maturation rate to observe cleavage rate, blastocyst rate and total cell number of blastocysts, after oocyte collection The results showed no significant differences observed in maturation rate with the nuclear development arrested in the MII stage (74.88 ± 2.00% vs. 81.144 ± 7.00%), after the culture for maturation. After insemination, the fertilized zygotes were continuously developed in the in vitro culture system, and cleavage were observed 48 hours after insemination. The two different oocyte collection methods (surgical vs. laparoscopic method), showed no difference in the cleavage rate (73.93 ± 1.90% vs. 68.30 ± 3.99%) and the blastocyst rate on the 7th day after fertilization (42.87 ± 1.26% vs. 42.65 ± 3.83%); however the surgical method had a higher total cell number of blastocysts (76.41 ± 1.29 vs. 69.00 ± 8.91). In this study, a laparoscopic oocyte pick-up technology was established with success. The blastocyst development capacity of the collected oocytes after in vitro culture was similar to that of surgical collect group. The laparoscopic oocyte pick-up technology can indeed be used for goat oocytes collection.

並列關鍵字

Goat ； Laparoscopic oocytes pickup ； Blastocyst rate