不同冷凍保護劑對紐西蘭白兔精子性狀之影響

本研究的目的在於比較不同兔精液冷凍保存步驟，以找出最適合的冷凍方法，提升解凍後精子之活動力（motility）、存活率（vitality）及精子前進值（progressive motility）等性狀表現。試驗將採樣自不同個體的兔精液先在5℃降溫後，以含有0.5% BSA（bovine serum albumin）與0.1 M或0.058 M sucrose之Tris-citrate-glucose （TCG）為基礎液，添加6、10或16% DMSO（dimethyl sulfoxide）、4% Ficoll70或10% LDL（low-density-lipoprotein）等不同組合冷凍稀釋液（extender）稀釋混合。再將此等精液混合液充填入0.25 mL之麥管在5℃中平衡45 min，然後放置於液態氮蒸氣（距液面5 cm處）進行冷凍降溫10 min，最後再移入液態氮中保存。冷凍解凍後以電腦輔助精子分析儀進行包含精子活力、精子存活率、精子前進值等性狀之分析。試驗結果顯示，最佳的兔精液冷凍操作條件為使用含10%DMSO作為冷凍保護劑，且以單一步驟冷凍法進行操作可以獲致解凍後於兔精子最佳活力（40.19 ± 7.65%）、存活率（31.45 ± 6.6%）及精子前進值（15.23 ± 2.77%）之結果。

關鍵字

冷凍保護劑；兔；精液冷凍保存

並列摘要

The aim of this study was designed to identify a suitable freezing protocol for rabbit semen by comparing the effects of different operating procedures. In our study, pooled semen was diluted 1:1 or 1:4 (v:v) with a freezing extender composed of Tris-citrate-glucose solution [containing 0.5% BSA (bovine serum albumin) and 0.1 M or 0.058 M sucrose] and 6, 10, or 16% DMSO (Dimethyl sulfoxide), 4% Ficoll70 or 10% LDL (low-density-lipoprotein) after cooling down to 5℃. The semen suspension was then loaded into 0.25 mL plastic straws and equilibrated at 5℃ for 45 minutes before freezing in liquid nitrogen vapor (5 cm above the liquid nitrogen surface). The sperm characteristics evaluated after thawing were sperm motility, vitality, and progressive motility. The best results of rabbit sperm freezing could be obtained by using 1:1 (v:v) dilution with the freezing extender consisting of 10% DMSO which contributed to the sperm characteristics after thawing of motility (40.19 ± 7.65%), vitality (31.45 ± 6.6%), and progressive motility (15.23 ± 2.77%).

並列關鍵字

Cryoprotectant ； Rabbit ； Semen cryopreservation

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