- 期刊
The Protects Effect of Breviscapine Against Hepatic Ischemia/Reperfusion Injury: Regulation of Endoplasmic Reticulum Stress and Apoptosis with Mitofusin 2
摘要
To investigate the effect of breviscapine against hepatic ischemia/reperfusion (I/R) injury and its underlying mechanisms in rats, 40 Sprague-Dawley male rats were randomly assorted into five groups (n=8 per group) as follows: Sham, I/R1 + normal saline (NS), I/R1 + breviscapine (Bre), I/R2 + NS and I/R2 + Bre, where label 1 and 2 denote ischemia time for 20 and 60 min, respectively. Bre or NS (10 mg/kg) was administered to the tail vein 1 h prior to surgery and immediately postoperatively. Reperfusion for 6 h, the blood and hepatic samples were collected immediately to evaluate hepatic function and histopathological changes, in addition with the expression levels of mitofusin 2 (Mfn2) and endoplasmic reticulum stress (ERS)-associated proteins. The results demonstrated that breviscapine decreased the elevated serum level of ALT and AST and ameliorated histopathological changes compared with the NS groups (P＜0.05). The expression of Mfn2 and Glucose-regulated protein 78 (GRP78) and Bcl2 protein were significantly increased in the Bre groups (P＜0.05), accompanied by decreasing the expression of C/EBP homologous protein (CHOP) and cleaved caspase-3 (P＜0.05) compared with the NS groups. These data revealed that breviscapine could ameliorate the hepatic I/R injury, possibly contribute to the up-regulation of Mfn2 expression to inhibit the ERS pathway, thus reducing the apoptosis of hepatocyte.
參考文獻
延伸閱讀
- Jalonen, U., Nieminen, R., Vuolteenaho, K., Kankaanranta, H., & Moilanen, E. (2006). Down-Regulation of Tristetraprolin Expression Results in Enhanced IL-12 and MIP-2 Production and Reduced MIP-3α Synthesis in Activated Macrophages. Mediators of Inflammation, 2006(), 220-227. https://doi.org/10.1155/MI/2006/40691
- 徐士閔（2014）。The Mechanism Study Underlying the Anti-oxidant and Anti-inflammation Activities of Ergostatrien-3β-ol from Antrodia camphorata Using Ischemia and Reperfusion Injury Rat Model〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2014.01011
- Lu, T. F. (2014). Involvement of Endoplasmic Reticulum Stress, Autophagy, and Apoptosis in Advanced Glycation End Products-Induced Mesangial Cells Injury [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2014.02617
- Gan, X., Su, G., Zhao, W., Huang, P., Luo, G., & Hei, Z. (2013). The Mechanism of Sevoflurane Preconditioning-Induced Protections against Small Intestinal Ischemia Reperfusion Injury Is Independent of Mast Cell in Rats. Mediators of Inflammation, 2013(), 153-164-114. https://doi.org/10.1155/2013/378703
- Yeh, C. H., Chen, T. P., Wang, Y. C., Lin, Y. M., & Fang, S. W. (2010). AMP-Activated Protein Kinase Activation during Cardioplegia-Induced Hypoxia/Reoxygenation Injury Attenuates Cardiomyocytic Apoptosis via Reduction of Endoplasmic Reticulum Stress. Mediators of Inflammation, 2010(), 25-33. https://doi.org/10.1155/2010/130636