Instrumental analysis is one of the core courses in universities, but the equipment is a few, so students always cannot practice the whole experiment procedure. In recent years, the popularization of virtual reality (VR) technology, VR has gradually appeared in various teaching research. From the perspective of Situation Theory, VR allows learners to interact with objects in the situation to construct knowledge or skills. This study uses the head-mounted virtual reality to develop and evaluate the HPLC instrument analysis-assisted learning system, to explore the feasibility of virtual reality aided instrument analysis experiments in teaching. The results of the open-ended questionnaire survey showed that the learning methods of virtual reality can help students to learn the experimental process and experimental operation of the instrumental analysis experiment.