海洋環保防污殺菌劑開發暨電化學技術之應用

Development of environment-friendly antifouling biocide and electrochemical applications

張介宇(Jie-Yu Chang)

指導教授 ： 李岳聯

國立臺灣大學/工學院/工程科學及海洋工程學研究所/碩士(2017年)

https://doi.org/10.6342/NTU201702467

摘要

本研究目的在探討金屬化合物作為殺菌劑之替代方案，降低防污塗料毒性及防止環境的重金屬汙染。藉由微生物實驗檢測化學藥品抑菌性，並進行電化學檢測，由電化學數據判斷細菌附著、生物膜形成機制及化學藥品抑菌性，最後使用光學顯微鏡、掃描電子顯微鏡及雷射共軛聚焦顯微鏡驗證實驗結果。
　　本研究第一部分為微生物實驗，首先抽取現地取樣樣品16S rNDA，利用變性梯度電泳分析(DGGE)出序列組成差異，經過序列比對後確定樣品中所含菌種。
　　菌種確定後，量測並繪出其原始生長曲線，加入本研究選用之殺菌劑，百里酚(Thymol)，測量添加不同濃度下對六種模式菌種生長抑制的效果，藉此得出此六種船殼底部生物膜之海洋細菌的最小抑制濃度(MIC，Minimum inhibitory concentration)。經濃度測試結果顯示，百里酚具有抑制細菌生長的效果，其最小抑制濃度約為125μg/L。
　　實驗第二部分為電化學檢測，藉由開路電位量測及交流阻抗檢測細菌是否附著及殺菌藥品抑制效果。由開路電位檢測結果顯示，當海洋細菌附著在底材表面將會造成電位不穩定震盪；使用交流阻抗分析，當金屬底材浸泡在含有海洋細菌的環境中，所得之底材阻抗與測量於純人工海水及添加殺菌劑進行殺菌之環境呈現相反趨勢。參考以上電化學實驗結果，便能夠評估細菌附著情形及抗菌藥品是否有效。並使用光學顯微鏡、掃描電子顯微鏡及雷射共軛聚焦顯微鏡，更加直觀地驗證微生物及電化學實驗，檢測生物膜的形成以及細菌附著造成底材腐蝕型態之改變。

關鍵字

海洋微生物 ； 生物腐蝕 ； 電化學 ； 開路電位 ； 交流阻抗

並列摘要

The aim of this study was attempted to find out new compounds instead of traditional biocide to decrease the toxicity of coating and prevent the heavy metal pollutions. Deteced the bactericidal activity of compound by microbial experimental, determined whether bacteria attached, biofilm formation and bactericidal activity by electrochemical experimental. Finally, Optical microsocope, SEM and CLSM were used to certifiy previous experiments.
The first part was microbial experiments, extracted 16S rNDA of sample which collected form Danshui, Taiwan and compared difference of sequence with database to determine the species of bacteria. After that, different concentrations of thymol were added into culture broth to find the bactericidal activity at different concentrations for six strains, draw the growth curves and determine the MIC(MIC，Minimum inhibitory concentration) of six strains. The results revealed that thymol had great bactericidal activity and its MIC was about 125μg/L.
The second part was electrochemical experiments, decteded the attachment of bacteria and bactericidal activity of thymol by open circuit potential (OCP) and Electrochemical impedance spectrum (EIS). The results of OCP In open-circuit potential, presence and attachment of bacteria causing potential oscillation, while the test environment added thymol and sterile electrolyte is more stably；In EIS analysis, we found that the trend of data is adverse in presence of bacteria and sterile environment.
According to these informations, the bactericidal activity and attachment of bacteria could be judge by electrochemical testing. OM, SEM and CLSM were used to observe the surface morphology and biofiom formation.

並列關鍵字

Marine microorganism ； biofouling ； electrochemical ； OCP ； EIS

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