仿生電活性奈米組裝分子多層之設計於高靈敏度C反應蛋白之電化學檢測

The Electrochemical Detection of high-sensitivity C-Reactive Protein Based on Biomimic Design of Electroactive Nanoassembly Multilayers

簡苓芝(Ling-Chih Chien)

指導教授 ： 林啟萬

國立臺灣大學/工學院/醫學工程學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.00084

摘要

電化學是對化學相關反應的研究，電化學法是一種快速簡便且成本低廉的方法，其特性可以運用在經過二茂鐵烷基硫醇分子修飾的電極來做為檢測蛋白質之感測端。本研究中以含二茂鐵尾端之烷基硫醇分子和脂質單層組成複合雙層仿生修飾電極，依此設計所製造出之工作電極以電化學檢測法用來作為仿生C反應蛋白感測器。此實驗之目的主要為驗證尾端含二茂鐵之烷基硫醇分子與含磷化膽鹼基之脂質單層所組成的仿生混合雙層膜是否可以執行C反應蛋白的檢測。不同莫耳分率之含二茂鐵分子溶液與無取代硫醇分子溶液混合所形成之自組裝單分子層可以操控其表面親疏水性及電子傳導性，尾端有氧化還原能力之二茂鐵分子層可以在分子辨識時提供分子內電子傳遞所需的能量。結果顯示浸泡含ferrocenylundecanethiol及undecanethiol之疏水性混合單分子溶液的金膜電極，對電極之電子傳遞能力較有幫助；且吸附磷脂質後，電極上之電活性分子會被阻礙，而引起電流的改變，則此仿生層之設計是可以用來量測C反應蛋白。其電化學性質和電極之電子傳遞能力也可以循環伏安法來定性，電流與濃度經線性迴歸後，其趨勢線與實驗資料之間的決定係數為0.8478。

關鍵字

C反應蛋白 ； 自組裝二茂鐵烷基硫醇分子 ； 循環伏安法

並列摘要

Electrochemistry is principally the study of chemical reactions. This feature makes the study of protein via ferrocene-terminated alkanethiol modified SAM a feasible plan. In this study, biomimic modified gold electrode with ferrocene-terminated alkanethiol and lipid complex layer is designed and fabricated to serve as biomimic C-reactive protein sensor using electrochemical determination. The scope of this research is to know whether ferrocen-terminated SAM and hydrogenated phosphocholine biomimic hybrid bilayer could be used to perform C-reactive protein detection. With different mole fraction of ferrocene-terminated alkanethiol mixed with unsubstituted alkanethiol to manipulate the surface hydrophilicity and electro-conductivity, this redoxactive layer may give appropriate electron transfer ability of interconversion of electrical energy during molecular recognition.
The result shows that mixed hydrophobic SAM, which consists of ferrocenylundecanethiol and undecanethiol formed by soaking gold substrates in ethanol solutions of these two compounds, can increase electrode electron transfer ability. And after coating phospholipids, the electroactive molecules are hindered from the electrode, thus this kind of biomimic layer design can be used to measure CRP. Its electrochemical property and the changes of electrode’s electron transfer ability are characterized by cyclic voltammetry. The output of linear regression between induced current and concentration shows a value of R squared of 0.8478 which implies a good linearity.

並列關鍵字

C-reactive protein ； self-assembled ferrocene-terminated alkanethiol ； cyclic voltammetry

參考文獻

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