不同高分子基材對HepG2細胞生長及細胞電泳之研究

陸瑞男(Jui-Nan Lu)

指導教授 ： 楊台鴻

國立臺灣大學/工學院/醫學工程學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00208

摘要

本實驗主要在探討將HepG2細胞株以體外培養的方式種於各種不同的高分子薄膜上，並利用MTT和LDH、細胞電泳、尿素和白蛋白的分泌、電子顯微鏡等工具來觀察細胞在這些不同機材上的生長、形態及弁鄐W的差異。
從實驗中我們發現細胞在聚乙烯醇（PVA）、幾丁聚醣上生長的情形並不理想，而在聚乙烯乙烯醇共聚物（EVAL），接枝半乳糖基的聚乙烯乙烯醇共聚物（GEVAL）、聚偏氟乙烯（PVDF）、尼龍66與組織培養皿卻生長的相當良好。在尿素及白蛋白分泌量的分析中GEVAL和那些原本長的不是很好的材料都能夠讓細胞擁有相當高的白蛋白分泌量，反而是那些長的相當好的基材細胞在上面所表現的弁酮蛪磲漁t。從細胞電泳的實驗中我們看到在有血清的環境下PVA及GEVAL上的細胞擁有著較高的運動性，一旦除去血清之後PVDF及GEVAL上的細胞反而擁有著較高的運動性。隨著Cycloheximide的添加之後每種基材上細胞的運動性表現變的皆差不多，最後從電子顯微鏡的觀察上顯示在幾丁聚醣、尼龍66、PVA及GEVAL上擁有著多層的生長形態，而在其它的基材上是處於單層的貼附。

關鍵字

尼龍66 ； 幾丁聚醣 ； 聚乙烯乙烯醇共聚物 ； 聚偏氟乙烯 ； HepG2細胞株 ； 聚乙烯醇

並列摘要

In this study, HepG2 was cultured on several substrates in vitro and was investigated the cell growth, function and morphology with the methods of MTT, LHD, electrophoresis, urea and albumin synthesis analysis and scanning electric microscopy.
In the experiment, HepG2 cell line could attach and spread well on the substrates of EVAL, galactosylated-EVAL, PVDF, Nylon66 and TCPS. However, PVA and chitosan weren’t good substrates for HepG2 to spread and growth. But in the experiment of assaying the quantity of urea and albumin in the culture medium, we found that galactosylated-EVAL and the substrates that cells didn’t grow very well could make the HepG2 cells to secret high quantity of albumin. And the substrates that made cell grow well would decrease the cell function of secreting the albumin. In the experiment of the electrophoresis, cells cultured on the PVA and galactosylated-EVAL substrates with the presence of serum have the higher mobility. But when we removed the serum from the medium, cells cultured on the PVDF and galactosylated-EVAL had the higher mobility relatively. With the addition with cycloheximide, the cells mobility on PVDF and galactosylated-EVAL decreased and was the same with the cell mobility on other substrates. SEM analysis of the HepG2 cultured on chitosan, Nylon66, PVA, galactosylated-EVAL showed the morphology to have the multi-layer structure. And cell morphology cultured on EVAL, PVDF and TCPS showed to be mono-layer structure.

並列關鍵字

HepG2 cell line ； PVDF ； EVAL ； Nylon66 ； PVA

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