轉型生長因子β誘導牙周韌帶細胞骨分化表現之影響

The influence of TGF-β on osteogenic differentiation in periodontal ligament cells

鍾享艗(Hsiang-Yi Chung)

指導教授 ： 郭彥彬

國立臺灣大學/牙醫專業學院/臨床牙醫學研究所/碩士(2012年)

https://doi.org/10.6342/NTU.2012.10109

摘要

牙周病的產生會破壞牙周組織的結構，減少牙齒周圍的支撐結構，最後導致牙齒的脫落，牙周病的治療從控制發炎及感染，近來擴展至再生治療的應用，以期恢復牙齒周遭的健康牙周組織。組織工程的進步，發展出許多生長因子的應用。TGF-β及CTGF，在人體或動物體中具有誘導骨再生的能力，。然而在不同組織具有相當的差異性。
研究目的：本研究探討在牙周韌帶細胞中，TGF-β及CTGF對牙周細胞骨再生能力的影響，了解這些生長因子對牙周組織的影響。並且探討TGF-β誘導CTGF訊息傳遞路徑。
材料與方法：本實驗利用牙周韌帶細胞，以TGF-β及CTGF去誘導細胞的骨分化，並檢測其基因表現及鹼性磷酸酶的活性。同時以TGF-β刺激牙周細胞，以西方墨點法檢測 CTGF的訊息傳遞路徑。
結果： TGF-β及CTGF會誘導牙周細胞骨分化基因 BMP2的表現。另外，TGF-β在牙周韌帶細胞中，會經由 JNK、Smad 3、Rac-1、及Src-family kinase 去誘導 CTGF的表現。
結論：TGF-β及CTGF會誘導牙周細胞骨分化基因 BMP2的表現，TGF-β同時會刺激CTGF的表現。因此，TGF-β可能會經由CTGF表現的路徑，去促進細胞的分化，誘導牙周組織的骨分化表現。

關鍵字：牙周再生，轉型生長因子β，結締組織生長因子

關鍵字

牙周再生 ； 轉型生長因子β ； 結締組織生長因子

並列摘要

Periodontal disease would led to destruction of periodontal tissue, reduce periodontal supporting structure, and finally led to tooth loss. The development of periodontal therapy starts from infection control to tissue regeneration in order to regenerate the healthy tissue surrounding tooth structure. Discovery from tissue engineering found many growth factors leading to tissue regeneration. Recently, transforming growthβ(TGF-β)and connective tissue growth factor (CTGF) can induce bone formation and osteoblast differentiation. However, previous studies show large variety among different cells and tissues
Objectivs: This study is design to find the influence of TGF-β and CTGF on the osteogenic differentiation of periodontal ligament cell,to understand the influence of these growth factors on periodontal tissue. Furthermore, we discover the signal transduction between TGF-β and CTGF to find the mechanism between two growth factors. Therefore, we want to realizing these mechanism and to apply these growth factors to clinical periodontal regeneration
Methods: Immortalized periodontal ligament cells (iPDL) received from Dr. Change of National Yang-Ming University. TGF-β and CTGF were applied to induce the osteogenic differentiation of iPDL. Gene expression was analyzed by Real-time PCR and the protein of differentiation was examined by alkaline phosphatase stain. Further, TGF-β was added to induced CTGF expression in iPDL. CTGF, phosphorylated Smad3, and phosphorlated JNK protein expression were analyzed by western blotting.
Results: TGF-β and CTGF can induce the osteogenic differentiation gene expression in iPDL. TGF-βalso can induce the alkaline phosphatase activity. Furthermore, in PDL, TGF-β can induce the expression of CTGF through JNK, Smad 3, Rac-1, and Src-family kinase.
Conclusion: TGF-β and CTGF can induce osteogenic differentiation in periodntal tissue. TGF-β also can induce CTGF expression in iPDL. Therefore, TGF-β might induce PDL osteogenic differentiation through CTGF expression.

Key words: periodontal regeneration, TGF-β, CTGF

並列關鍵字

periodontal regeneration ； TGF-β ； CTGF

參考文獻

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