阻力訓練與補充骨骼健康食品對化療小鼠骨質疏鬆之緩解影響

過去針對骨質疏鬆之研究多著重於老化族群，而癌症病患因化療藥物導致之骨質疏鬆問題則經常被忽略；多項研究指出Doxorubicin化療藥具造成骨骼損傷之實證，若化療期間進行阻力訓練和攝取增益骨骼之健康食品，則可能保留其骨質密度。目的：本研究選擇安全性高之低強度阻力訓練和骨骼健康食品的介入，探討是否能延緩化療藥物造成之小鼠骨質疏鬆的症狀。方法：本研究使用20隻8週齡健康ICR小鼠進行為期三週的實驗，隨機分為控制組、化療組、化療運動組、化療食品組、化療運動食品組。需注射化療藥之組別，間隔兩天施打共四次、累積劑量至每公斤小鼠16毫克；使用自製紙板階梯進行每週三次，共九次的低強度阻力訓練；健康食品內含龜板、鹿角、葡萄糖胺和軟骨素，每日以管餵提供每公斤小鼠100毫克的劑量。實驗後犧牲小鼠取其股骨，利用微電腦斷層掃描儀取得三維掃描影像和骨骼參數，以單因子變異數分析檢測實驗結果中骨質流失和緩解的狀況。結果：根據小鼠三維掃描影像及各骨骼數據之統計分析結果，對小鼠施打化療藥後造成了明顯的骨質流失，僅進行阻力訓練和攝食骨骼健康食品的化療小鼠則沒有與化療小鼠有顯著骨質保留的結果，而在阻力訓練與骨骼健康食品搭配介入下的化療小鼠在統計上與其他化療小鼠組別皆有骨骼保留之顯著差異。結論：同時進行阻力訓練和攝食健康食品對於延緩化療造成之骨質疏鬆是具可預期性的，僅有阻力訓練的組別可能需再提高強度方能有保留骨質的效用，若提高骨骼健康食品之攝取含量，對於化療小鼠之骨骼保健應有更明顯的作用。

關鍵字

肌力訓練；健康食品；骨質密度；化學治療；癌症

並列摘要

The aging population was typically the subject of research in previous studies on osteoporosis, while cancer patients who suffer from chemotherapy-induced osteoporosis were often overlooked. Several studies have shown evidence of bone damage from Doxorubicin chemotherapy, and that bone density may be preserved if resistance training and bone health foods are intervened. Objective: This study opted to introduce safe, low-intensity resistance training and bone health foods, to investigate whether they could delay the loss of bones caused by chemotherapy drugs in mice. Methods: Twenty 8-week-old healthy ICR mice were randomly divided into five groups for a three-week experiment: (1) control, (2) chemotherapy, (3) chemotherapy with resistance training, (4) chemotherapy with bone health foods, and (5) chemotherapy with resistance training and bone health foods. The chemotherapy group was administered chemo injections four times at two-day intervals at a cumulative dose of 16 mg/kg of mice body weight. Low-intensity resistance training was performed three times a week using a handmade cardboard ladder as equipment for a total of nine sessions. Bone health foods including turtle plate, deer antler, glucosamine, and chondroitin were administered by tube daily at a dose of 100 mg/kg of mice body weight. After the experiment, the microcomputer tomography method was used to obtain 3D scanned images and skeletal parameters of femurs excised from mice. Results: Based on the 3D scans of the mice and the statistical analysis results of skeletal data, the administration of chemotherapy drugs to the mice resulted in significant bone loss. The study also showed that there was a statistically significant difference in bone retention between mice that received both resistance training and consumed bone health foods, and other groups without. On the other hand, mice that received chemotherapy along with either resistance training or consuming bone health foods did not show significant bone retention, compared to the results of the group of mice that only received chemotherapy. Conclusion: Concurrently receiving resistance training and maintaining healthy food intake predictably results in the delay of chemotherapy-induced osteoporosis. The group that only received resistance training may need to increase training intensity to amplify the effect of bone preservation. Increasing the intake of bone health foods should further promote the bone health of mice that went under chemotherapy.