藉由泰勒庫埃特流優化血小板回收率

Platelet Recovery Rate Optimization Using Taylor-Couette Flow

陳聖儒(Sheng-Ju Chen)

指導教授 ： 盧彥文

國立臺灣大學/生物資源暨農學院/生物機電工程學系/碩士(2020年)

https://doi.org/10.6342/NTU202001341

摘要

本研究建立了泰勒-庫埃特裝置並將其用於血液分離以製備血小板濃縮液。由於血液中工作流體的複雜性，設備需要進行系統分析以評估和優化其過濾效率。首先應用流體視覺化技術在操作過程中從泰勒裝置中抽取血球細胞以了解不同泰勒-庫埃特流態的細胞分類能力。泰勒渦流對不同大小的血細胞進行了分類：較大的血球細胞向渦流的外圍移動，而較小的血球細胞保持靠近渦流的中心。
此外，我們還使用兩種不同的裝置設計和一系列操作參數（包括轉速和流速）來評估和優化血液過濾效率。發現泰勒渦流可對血球細胞進行分類並提高過濾效率。 Ω = 1500 rpm時，PLT的回收率約為40％，體積比為40％，而RBC和WBC的回收率分別為75％和85％。

關鍵字

泰勒庫埃特流 ； 軸流效應 ； 血球細胞分類 ； 回收率 ； 濾餅層

並列摘要

A Taylor-Couette device is developed and utilized in blood separation to prepare platelet concentrate. Due to the complexity of the working fluids in blood, the devices require systematic analysis to evaluate and optimize their filtration efficiency. The flow visualization techniques and cell extraction from the device during the operation for the size distribution were first applied to understand the cell classification capability of different Taylor-Couette flow regimes. Blood cells at different sizes were classified by Taylor vortices: larger blood cells moved toward the periphery of the vortices, while smaller blood cells remained close to the center of the vortices. The blood filtration efficiencies are then evaluated and optimized with two different device designs and a series of operation parameters, including rotational speed and flow rates. It is found that Taylor vortices classify the blood cells and enhance the filtration efficiency. An approximately 90% recovery rate for PLT at rotational speed=1500 rpm within 40 % volumetric ratio were obtained, while the ones for RBC and WBC were 75% and 85% respectively.

並列關鍵字

Taylor Couette flow ； axial flow effect ； recovery rate ； blood cell classification ； filter cake

參考文獻

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