奈米流道生物感測研究

Biosensors in Extended Nanofluidic Device

林以立(Yii-Lih Lin)

指導教授 ： 周家復

共同指導教授 ： 李弘文(Hung-Wen Li)

國立臺灣大學/理學院/化學研究所/博士(2016年)

https://doi.org/10.6342/NTU201602108

摘要

微流道具有低樣品體積需求、反應速度快、微型化與自動化的優勢，已被廣泛應用於生物醫學檢驗。近年來技術的進展，更將流道的尺度推到奈米等級，促使生物分子間的反應距離更接近其擴散距離，達到極高(接近100%)的反應效率。本研究結合表面修飾與室溫晶片封裝方法，建立了一個結合抗體陣列的奈米流道生物感測平台。藉由螢光標記，即時觀察奈米流道內抗原與抗體的結合反應。由於奈米流道內的高反應效率特性，常見上游的抗體陣列抓取了多數的螢光抗原，造成下游生成無螢光訊號之無反應區域。我們模擬流道內的流體輸送與反應，重現奈米流道內反應的空間分佈，並開發了奈米流道內特用的一維模型，簡化並加快運算的效率。本研究可望進一步開發為微小化、同時檢測多種生物標記之檢驗平台。

關鍵字

奈米流道 ； 微流道 ； 生物感測 ； 反應動力

並列摘要

Nano/microfluidic devices have shown their superior capabilities in various applications. As compared to conventional biochemical assays, nano/microfluidic devices pose minimized dimensions, reduce the required sample consumption and shorten the overall assay time. Recent progress in lab-on-a-chip technologies further allows the miniaturization of fluidic channels to nanoscale level where molecular reactions become extremely efficiently as the inter-molecular distance are confined to be comparable to the molecular diffusion distance. This study represents a methodology to implement antibody microarray into extended nanoslits. Through moderate surface modification, fluidic chips are compatible with microarray spotting and the following chip encapsulation procedure. A room temperature chip-bonding process using polysilsesquioxane as gasket layer was integrated in the procedure to preserve the activities of the immobilized biomolecules. Furthermore, the antigen-antibody binding kinetics in the confined space was observed via real-time fluorescence imaging. In addition to antibody specificity preservation, extremely high reaction efficiency was observed as upstream microspots collect most fluorescently labeled target molecules and creates a dark depletion zone in the downstream. The kinetics was simulated through a finite element model and also a simplified one-dimensional convection-reaction model, which confirms that diffusion is efficient in the confined space. This work provides a combined strategy to incorporate protein microarrays within extended nanoslits, and a side-by-side simulation to study the kinetics.

並列關鍵字

nanofluidics ； microfluidics ； biosensor ； reaction kinetics

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