- 學位論文
異源表現綠竹 BoMSP41 對於阿拉伯芥生長之影響
Effects of ectopic expression of BoMSP41 on the growth of Arabidopsis thaliana
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摘要
BoMSP41 (Bambusa oldhamii monocot specific protein-41) 為綠竹中未知功能 基因，其基因表現量於綠竹快速生長時期的節間被大量提升，推測其可能參與綠竹生長。本研究欲探討 BoMSP41 之功能，故建立帶有 FLAG-BoMSP41 或 BoMSP41-FLAG 的轉殖阿拉伯芥 (Arabidopsis thaliana)，觀察異源表現 BoMSP41 對於轉殖株生長之影響。觀察發現轉殖株相對於野生型的開花時間較晚、株高較高且於高濃度的葡萄糖下，其發芽率較低。推測 BoMSP41 可能參與阿拉伯芥的開花、細胞增生或延長以及種子發芽。
並列摘要
BoMSP41 (Bambusa oldhamii monocot specific protein-41) is an unknown function gene which is greatly up-regulated in the internodes of rapid growing bamboo, suggesting that BoMSP41 may involve in bamboo growth. To gain insight into the function of BoMSP41, Arabidopsis transgenic plants overexpressing Flag-BoMSP41 or BoMSP41-FLAG is established. The effect of ectopic expression of BoMSP41 on the transgenic plants was investigated. Comparing to the wild type, the flowering time was delayed and the plant height was increased in the transgenic Arabidopsis. Additionally, the germination rate of the transgenic plant was decreased under high concentration of glucose. The results suggest that BoMSP41 may involve in the flowering, cell proliferation and/or elongation, and seed germination.
參考文獻
張皓鈞 (2012) 綠竹中 BoMSP41基因之分子選殖與檢定，碩士論文，國立臺灣大學生命科學院生化科技學系。
李佩儒 (2014) 重組綠竹 Monocot-Specific Protein-41 之純化與性質檢定，碩士論文，國立臺灣大學生命科學院生化科技學系。
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