山菅蘭根部之化學成分研究

Studies on the chemical constituents of the roots of Dianella ensifolia(L.) DC.

蘇聖涵(SHENG HAN, SU)

指導教授 ： 郭悅雄

國立臺灣大學/理學院/化學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.00458

摘要

山菅蘭Dianella ensifolia(L.) DC.分布在廣東、廣西、福建、日本、琉球、台灣、澳洲、夏威夷群島等地。海拔1500公尺以下地區或近郊，全株的汁液有毒，但可將其搗爛外敷，根莖為外用治瘡癬。酒蒸外用，治跌打損傷。
山菅蘭根部，以甲醇浸泡，萃取兩次，過濾並合併成濾液，經減壓濃縮後得376公克。萃取物以乙酸乙酯和水進行分配萃取，得乙酸乙酯層濃縮物90公克，運用矽膠薄層分析、高效液相層分析儀及再結晶等分法進行一步的分離純化。單離以後的化合物，則利用紅外線光譜、氫核磁共振光譜、碳核磁共振光譜及質譜做解析，並輔以二維核磁共振光譜(2D-NMR)，進行結構的確認。

本實驗共鑒定得到47個化合物，分別為：
一、類黃酮類(Flavonoids)化合物15個
二、查耳酮類(Chalcones)化合物4個
三、色酮類(Chromones)化合物2個
四、芳香族類(Aromatics)化合物16個
五、木酚素類(Lignoids)化合物1個
六、類固醇類(Steroids)化合物7個
七、其他類(Others)化合物2個

其中6個為新化合物，3個為從天然物中首次分離得到的化合物。

關鍵字

山菅蘭根部

並列摘要

Dianella ensifolia(L.) DC., distributed over Fukien, Taiwan, Japan and Aussie, was found below altitude of 1500 meters. Its sap is poisonous. However, the smashed mixture of its stems and roots can be directly used as medicine for treating tinea, it can be further used for treating bruses.
The leaves of Dianella ensifolia(L.) DC. were extrated with methanol. The extract was concentrated in vacuo to yield a residue which was suspended in water, and this phase was partitioned with ethyl acetate. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography and high performance liquid chromatography (HPLC) to give 47 compounds. These compounds are classified to 15 flavonoids, 4 chalcones, 2 chromones, 16 aromatics, 1 lignoids , 7 steroids, and 2 others. Of these, 6 new compounds and 3 compounds isolated for the first time from natural sources
The known compounds were identified by spectral analysis and by comparsion with those reported in literatures or from authentic samples. The structures of new compounds were elucidated by spectral evidence including 1D and 2D NMR techniques.

並列關鍵字

roots of Dianella ensifolia(L.) DC.

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