Cellulose is an insoluble dietary fiber and is the most abundant polysaccharides in nature. It has been proved that cellulose can improve human health, such as promoting gastrointestinal peristalsis, reducing weight, preventing constipation and colon cancer. It has been reported that macrominerals binding capacity of fiber is related with particle size and water holding capacity. Volume mean diameter of cellulose was significantly reduced by media-milling, resulted in both the reducing particle size and molecular weight. For example, the molecular weight was reduced from 646.29 kDa to 117.1 kDa after 90 min media-milling. Via sediment method, the suspended cellulose yield a zeta potential of -16.6 mV, a negative charge. After media-milling, the absolute value of zeta potential was increased, indicates that nano/submicron cellulose dispersion is more stable, Isoelectric point(IEP) of cellulose raw material is pH 3.03, and dropped to 2.74 after 90 min media-milled, shows that after media-milling, cellulose particles are more negative-charged. Media-milling also improves WHC of cellulosic particles. By centrifugation method, WHC increased 7.9 fold after 30 min media-milling, but decreased after 30 min. Viscosity of cellulose dispersion increased first from 1.71 cp to 25.46 cp and then decrease after 30 min. WHC and viscosity were found correlated. Media-milling can also enhance Mg, Ca, Na, K binding capacity with a relationship with WHC.