以口咽吸入多次暴露柴油引擎微粒對小鼠誘發神經毒性之研究

現今的空氣污染日益嚴重，空氣污染的暴露也會造成許多身體健康上的影響。研究指出暴露於空氣污染之中會造成呼吸系統、心血管疾病甚至影響中樞神經系統(central nervous system)。直至今日，大氣細懸浮微粒與神經退化性疾病(neuro-degenerative diseases)的關聯已經被確立了，然而其中的詳細機制仍舊不得而知。現今大都市中的空氣污染排放嚴重，柴油引擎微粒(diesel exhaust particles, DEPs)更是主要的污染物之一。柴油引擎微粒的神經毒性可能與神經退化性疾病的產生有所關聯。因此，我們希望藉由實驗動物模型暴露於柴油引擎微粒來觀察實驗動物是否在空間記憶、學習能力上有所改變。同時，我們也會蒐集與測量動物的腦部氧化壓力(oxidative stress)、腦中神經纖維纏結指標，以了解柴油引擎微粒對中樞神經系統的神經毒性。本研究使用32隻C57BL/6母鼠，透過口咽吸入(oropharyngeal aspiration，OA)在三週內分6次暴露共300 μg的SRM 1650b型柴油引擎微粒。柴油引擎微粒購買自美國國家標準暨技術研究院，是由多種重型柴油引擎機具燃燒所排放出來的引擎微粒，適合代表使用柴油引擎的重型機具與車輛所產生的微粒。在暴露之後，其中一批小鼠會在24小時內犧牲(急性組)；另一批則會給予3個月的恢復期(恢復組)並且進行莫式水迷津(Morris water maze)、被動迴避測驗(passive avoidance test)兩個動物行為實驗。小鼠犧牲後會取下全腦做為樣本並再分為小腦、海馬迴、大腦皮質三個腦區進行研究。這些樣本將會使用LC-MS/MS分析腦中脂質過氧化(lipid peroxidation)的指標丙二醛(malondialdehyde, MDA)，也會使用西方點墨法(Western blot)檢測神經纖維纏結指標：total Tau、phosphorylated Tau蛋白。部分腦組織灌流後進行石蠟包埋、H&E染色與切片，透過專業獸醫師分析組織病理。實驗結果表示，脂質過氧化指標MDA於急性組的暴露組小腦、海馬迴有顯著的上升，在其他組別與腦區則無差異；神經纖維纏結指標分析上發現，total Tau、phosphorylated Tau蛋白的表現量在急性組的暴露組大腦皮質顯著的高於控制組，其他組別與腦區同樣無差異。動物行為實驗的結果表示，恢復前與恢復後的莫式水迷津與被動迴避試驗控制組與暴露組間皆無差異。在病理切片的部份，在兩大組別的暴露組肺部有發現微粒的堆積與發炎反應的產生，不過腦部並沒有顯著的發炎與病變發生。總結來說，急性暴露於柴油引擎微粒的神經毒性會造成腦部的氧化壓力、total Tau與phosphorylated Tau蛋白表現量上升，但是經過長期的恢復後並沒有在腦部發現任何病變與生物指標上升而且在動物行為實驗上也沒有差異。這種結果可能表示小鼠在三個月恢復期中，由於自身的清除機制消除柴油引擎微粒產生的神經毒性。再加上本次使用健康的成年小鼠暴露，比起易感受族群(嬰幼兒、年長者)更不易受到神經毒性的傷害且恢復快速。不過，本實驗也指出急性的柴油引擎微粒暴露能夠造成中樞神經系統的受損，若長期暴露之下所帶來的危害更不容小覷。本次實驗在建立完整的柴油引擎微粒毒性影響中樞神經系統機制仍缺乏部份證據來佐證，期望未來可以增加檢測自噬作用、乙型類澱粉蛋白亦或更多的相關生物指標或在實驗動物上使用基因轉殖動物達到完整的結果。

關鍵字

柴油引擎微粒；口咽吸入；神經毒性；神經退化性疾病；氧化壓力；中樞神經系統；神經纖維纏結

並列摘要

The association between ambient particles and neurodegenerative diseases has been acquired. However, the exact mechanisms remain unclear. Diesel exhaust particles (DEPs) are one of major sources of ambient particles and it is also related to neuro-degenerative diseases. Here, we investigated if DEPs’ neurotoxicity induced behaviour changes of learning and memory of mice. We also evaluated the level of oxidative stress and Tau protein expression in brain tissue of mice. Female C57BL/6 mice were administered with 300μg of SRM 1650b DEPs in 3 weeks through multiple oropharyngeal aspiration (OA). SRM 1650b DEPs used in this study was purchased from National Institute of Standards and Technology. After exposure, one group was sacrificed at 24 hours, and the other group was allotted 3-months recovery time. Morris water maze test was conducted to study the spatial learning and memory of mice. Additionally, passive avoidance task was used to evaluate learning and memory. The cortex, hippocampus and cerebellum regions were sampled after the sacrifice. Lipid peroxidation was measured by assaying the malondialdehyde level with LC-MS/MS. As markers of neurofibrillary tangle, expression of total Tau protein and phosphorylated Tau protein were also tested by western blot. Brain tissues were stained by hematoxylin and eosin (H&E) for histopathology. Our results showed that MDA concentration significantly increased in hippocampus and cerebellum of mice (p<0.01, Wilcoxon rank sum test), which sacrificed right after exposure in 24 hours. We also found that expression of total Tau and phosphorylated Tau protein were significantly higher in the cortex of the exposure than the control (p<0.05, Wilcoxon rank sum test) in the same group. Whereas, we didn’t find any noticeable increasing of MDA concentration and expression of Tau protein in 3-months recovery group. The results of the Morris water maze and passive avoidance test didn’t show any difference between control and exposure, either. The histopathology of 3 brain regions (olfactory bulb, hippocampus and cerebellum) had no histopathological changed, but mild inflammation in lung of exposed mice in both groups. Previous reports show that DEPs caused an increase of oxidative stress and expression of Tau protein, but there was no deficit of spatial learning and memory of animals. In our study, we only found that DEPs induced oxidative stress up-regulation and higher expression of Tau protein in the CNS, but a spatial learning and memory defect after acute exposure were not observed. Therefore, we conclude that acute exposure to DEPs could cause damage to the CNS and 3-months of recovery time could nearly remove the neurotoxicity of DEPs. However, the underlying mechanism through which DEPs affects our CNS requires further studies including biomarkers of autophagy (LC3b), beta-amyloid (Aβ), and microglia activation (Iba-1). In conclusion, acute exposure to DEPs could cause neurotoxicity in mice. Our study also indicated a possible link between neurotoxicity of DEPs and neurodegenerative diseases. However, further studies are needed to elucidate the relationship between DEPs and neurodegenerative diseases.

並列關鍵字

diesel exhaust particles (DEPs) ； oropharyngeal aspiration (OA) ； neurotoxicity ； neurodegenerative diseases ； oxidative stress ； central nervous system (CNS) ； neurofibrillary tangle

參考文獻

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以口咽吸入多次暴露柴油引擎微粒對小鼠誘發神經毒性之研究

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