小孢子靈芝免疫調節蛋白抑制泌尿上皮癌細胞生長及調控其細胞程序性死亡機制探討

Ganoderma microsporum immunomodulatory protein inhibits proliferation and modulates program cell death in urothelial carcinoma cells

黃聖元(Sheng-Yuan Huang)

指導教授 ： 許瑞祥

國立臺灣大學/生命科學院/生化科技學系/碩士(2014年)

https://doi.org/10.6342/NTU.2014.10330

摘要

泌尿上皮癌細胞（urothelial carcinoma cells）的種類有95%都是移行性上皮癌細胞（transitional cell carcinoma），而此類的癌細胞不論在化學治療或是免疫治療，都會有很高的復發機率。小孢子靈芝蛋白的免疫調節功能已經廣為人知，但是其被運用在抗癌治療的研究相對很少而且機制未明。本研究利用兩株不同的膀胱癌細胞株，BFTC-905及TSGH-8301為體外模式，以驗證小孢子靈芝蛋白對癌症之生長抑制，及對後續所引發之程序性細胞死亡進行探討。本研究證實，小孢子靈芝蛋白能夠抑制兩株受試膀胱癌細胞的生長，並且引發半胱天冬酶相關之自凋亡之程序性死亡反應。而兩株細胞的反應差異， TSGH-8301對GMI蛋白質的耐受度較BFTC-905強之原因，為能夠誘發自噬作用的保護作用與否。本研究期待能夠利用此蛋白質的免疫調節及抗癌功能於治療膀胱癌之轉譯醫學。

關鍵字

膀胱癌 ； 移行性上皮癌細胞 ； 靈芝 ； 小孢子靈芝免疫調節蛋白質 ； 程序性細胞死亡 ； 轉譯醫學

並列摘要

At least 95% of urothelial carcinoma cells belong to transitional cell carcinoma. Chemotherapy or immunotherapy on bladder cancer both suffer from poor prognosis. While the functions of immunomodulatory proteins in Ganoderma are widely known, the knowledge of their therapeutic effects toward tumors remains scarce. In this study, we apply Ganoderma microsporum immunomodulatory protein to two different bladder cancer cell lines, BFTC-905 and TSGH-8301, to explore its effect on cell proliferation and the behind mechanisms of cell death. GMI inhibits the proliferation of both cell lines and induces apoptosis. The distinctive tolerance toward GMI on two bladder cancer cell lines is suspected to whether GMI could induce the protective mechanism, autophagy or not. We believe this novel therapy can shed new light on the studies of translational medicine toward bladder cancer.

並列關鍵字

Bladder cancer ； transitional cell carcinoma ； Ganoderma ； Ganoderma microsporum ； immunomodulatory protein (GMI) ； program cell death ； translational medicine

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