甲魚粉酵素水解產物之降血壓作用

Antihypertensive effect of enzymatic hydrolysates from soft-shelled turtle powder

張譽薰(Yu-Hsun Chang)

指導教授 ： 江文章

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.00851

摘要

甲魚 (soft-shelled turtle,SST) 為中國傳統藥材，具有多種生理功效。本實驗研究目的為，利用甲魚粉經木瓜酵素水解製備，具有調解血壓功效的甲魚粉酵素水解產物。甲魚粉經反應曲面法二因子 (酵素添加量、水解時間) 旋轉式中心組合設計，利用胜肽濃度、 ACE 抑制活性為評估條件，發現 2% 甲魚粉懸浮液，在 pH 5.5 、 60℃ 、 5% 木瓜酵素、 3 小時水解時間，為較佳水解條件。未經水解的甲魚粉懸浮液其 ACE 抑制活性低， IC50 為 16.2±1.6 mg/mL ，經木瓜酵素水解得到的甲魚粉酵素水解產物 ACE 抑制活性明顯提高，IC50 為2.87±0.32 mg/mL。
在短期動物實驗中，以甲魚粉酵素水解產物 500 mg/kg BW 劑量單次管餵自發性高血壓大鼠 (spontaneously hypertension rat, SHR) 後，於管餵後5小時，其收縮壓顯著性下降；長期動物實驗中，以含 0.6% 甲魚粉酵素水解產物的飼料餵食，於第 4、6 週時，收縮壓較控制組顯著性下降，於第8週時收縮壓則極顯著下降，餵食甲魚粉酵素水解產物的 SHR 血漿中 ACE 活性顯著性低於控制組。綜合上述，甲魚粉經木瓜酵素水解後的水解產物具有調解血壓功效。

關鍵字

甲魚 ； 血管收縮素轉換酶木瓜酵素 ； 高血壓 ； 反應曲面法 ； 活性胜肽

並列摘要

Chinese soft-shelled turtle (SST) is a traditional Chinese medicine, and was also confirmed that possessed various kinds of physiological functions. The objectives of this study were to produce anti-hypertension effect of SST powder hydrolysate digested by papain. SST powder was hydrolyzed under various conditions of hydrolysis time and enzyme-to substrate ratio (E/S, %) according to a response surface methodology (RSM) 2 factors rotatable central composite design, and hydrolysate were evaluated by peptide concentration and ACE inhibitory activity. The better hydrolysate conditions of SST powder hydrolysate digested by papain are pH 5.5, 60℃, 5% E/S for 3 hours. The SST powder showed limited IC50 value of ACE inhibitory activity were 16.2±1.6 mg/mL. The SST powder hydrolysate derived from hydrolysis of papain obviously increased ACE inhibitory activity, and IC50 were 2.87±0.32 mg/mL.
In short term animal experiment, single oral administration of 500 mg/kg BW of SST powder hydrolysate to spontaneously hypertension rat (SHR) was significantly lowered systolic blood pressure at 5th hours. In long term experiment, the experiment group feed diet containing 0.6% SST powder hydrolysate was noticeable decreased systolic blood pressure compared to control group at 4th and 6th weeks (p<0.05) and significant suppression at 8th weeks (p<0.01). The ACE activity of SHR feed diet containing 0.6% SST powder hydrolysate were lower than control group. The results suggested that SST powder hydrolysate digested papain can modulate blood pressure.

並列關鍵字

Soft-shelled turtle ； angiotensin I-converting enzyme ； papain ； hypertension ； response surface methodology ； bioactive peptide

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