薏仁麩皮乙醇萃取物及其區分物貯存期間之品質變化

The Quality Change of Adlay Bran Ethanolic Extract and its Different Fractions During Storage Period.

林如翠(Ju-Tsui Lin)

指導教授 ： 江文章

國立臺灣大學/生物資源暨農學院/食品科技研究所/碩士(2009年)

https://doi.org/10.6342/NTU.2009.00856

摘要

體內長期發炎目前已證實與癌症、氣喘和動脈粥狀硬化症等諸多慢性疾病息息相關。薏苡（Coix lachryma-jobi L.var. ma-yuen Stapf）自古以來即為食藥兩用之材料，許多研究也均顯示其具有增強免疫力、調節賀爾蒙、抗氧化、抗發炎、抑制腫瘤等生理功效。為促使此保健食材能獲得最廣泛之應用與效用，因此，本實驗以評估薏苡成為保健食品之觀點為研究方向，並參照行政院衛生署健康食品安定性試驗之模式執行半年期，並於時間點 0、3、6 個月或 0、1、2、3、6 個月，來探討薏仁麩皮乙醇萃取物（adlay bran ethanolic extract, ABE）及其區分物 ABE-Hex、ABE-Ea、ABE-Bu 中具抗發炎生理活性物質於試驗期間的變化，其中包含定性與定量 vanillic acid、caffeic acid、p-coumaric acid、ferulic acid、syringaldehyde、6-methoxy-2-benzoxazolinine、4-hydroxyacetophenone、chlorogenic acid 等 8 個酚類化合物，以及 campesterol、stigmasterol、β-sitosterol 等 3 個植物固醇化合物。同時，於安定性試驗過程中亦配合測定品質指標過氧化價（peroxide value, POV）來評估各樣品貯存的安定性。於實驗結果顯示，ABE 和 ABE-Hex 之 POV 在加速試驗第 0 個月時較其他區分物為高，且有隨貯存時間的增長而下降之趨勢；ABE-Ea 則於第 0 個月時 POV 較其他區分物為低，且有隨貯存時間的增長而增加之趨勢；ABE-Bu 其 POV 之變化則較為穩定，各時期的 POV 均呈現較低之情形。於酚類化合物之定性與定量的數值顯示，ABE 和其區分物均含有豐富的酚類物質，且所有區分物中以 ABE-Ea 和 ABE-Bu 之含量為最多，而 ABE-Hex 的含量為最少，且經安定性試驗後，所有樣品所含的酚類化合物均有隨貯存時間的增加而有消長之現象發生，且於安定性試驗進行至第 6 個月時，所有的酚類物質均有大幅下降之情形發生。於植物固醇化合物之定性與定量的數值顯示，各區分物均以 β-sitosterol 含量較高，且所有區分物中以 ABE 和 ABE-Hex 之含量為最大，而 ABE-Bu 的含量為最少。經安定性試驗後，所有樣品中的植物固醇化合物均有隨貯存時間的增加而有下降之現象發生，且於安定性試驗進行至第 6 個月時，所有的植物固醇化合物均呈現大幅下降之情形。綜合上述，ABE 及其區分物之樣品在安定性試驗之環境中，其生理活性物質均有波動之趨勢，因此，若未來欲發展以此樣品為產品之保健食品，應特別注意其儲存環境，以確保其品質。另本次研究所定性與定量之酚類與植物固醇化合物可能為貢獻所有樣品具抗發炎功效之主要成分物質，且 ferulic acid 亦可能為 ABE-Ea subfractions 具良好抗發炎功效之指標性化合物質。

關鍵字

薏苡 ； 抗發炎 ； 酚類化合物 ； 植物固醇化合物

並列摘要

Long-term inflammation has been proven that is related to chronic diseases such as cancer, asthma, and atherosclerosis. Adlay (Coix lachryma-jobi L var. ma-yuen Stapf) was used as food and medicine, and many researches pointed out that adlay possess immuno-mediating, hormone-regulating, anti-oxidative, anti-inflammation, and anti-cancer effects. To promote functional materials utilized comprehensively, this study supposed adlay as a nutritional supplement to evaluate the stability according to Health Food Management Law published by Department of Health. In 6 months experimental period, time points at 0, 3, and 6 month or 0, 1, 2, 3, and 6 month, the samples of ABE (adaly bran ethanolic extract) and sub-fractions (ABE-Hex, ABE-Ea, and ABE-Bu) were taken to quantify the changes of components possessed anti-inflammation activity during storage period. 8 phenolic compounds and 3 phytosterols were chosen as targets, the former comprised included chlorogenic acid, vanillic acid, caffeic acid, p-coumaric acid, ferulic acid, 4-hydroxyacetophenone, syringaldehyde, and 6-methoxy-2-benzoxazolinine, while the latter comprised campesterol, stigmasterol, and β-sitosterol. Meantime, peroxide value (POV) was used to evaluate sample stability during storage periods. The results showed that POV in accelerating test were higher in ABE and ABE-Hex than other fractions at 0 month, and became lower as storage time passed. POV in ABE-Bu was stable and low at all time points. Phenolic compounds are abundant in ABE and sub-fractions, which located at ABE-Ea and ABE-Bu, are the most among all groups. As storage time passed, phenolic compounds contents changed in all fractions, and after 6 month all of the phenolic compounds significantly decreased. Values in phytosterols pointed out that β-sitosterol occupied the most quantity in all fractions, while ABE and ABE-Hex contained most phytosterols. After storage periods phytosterol contents decreased and at the time point 6 month the contents lowered significantly. Above all, the active compounds in ABE and fractions changed as storage time passed by, so the storage environment shall be noticed that to ensure quality of extracts. Still, qualified and quantified compounds in this study are active compounds that possess anti-inflammatory effect and ferulic acid may be mainly compound in ABE-Ea subfractions contributed to anti-inflammation.

並列關鍵字

adlay seed ； anti-inflammation ； phenolic compounds ； phytosterol compounds

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