水體碳氮比對益生菌及副溶血弧菌生長、水質與相互抑制之影響

Effects of C/N ratios on Growth, Water Quality and Mutual Inhibition of Probiotic and Vibrio parahaemolyticus

高岱筠(Tai-Yun Kao)

指導教授 ： 陳秀男

國立臺灣大學/生命科學院/漁業科學研究所/碩士(2015年)

https://doi.org/10.6342/NTU.2015.00838

摘要

本實驗將糖蜜作為碳源添加至養殖水中，使水體碳氮比分別為0, 5, 10, 15, 20五種比例，0為無添加碳源組別，作為控制組。探討不同碳氮比條件對養殖環境中枯草桿菌 (Bacillus subtilis)、光合菌 (Rhodobacter sphaeroides) 及副溶血弧菌 (Vibrio parahaemolyticus) 生長、水質處理及相互抑制之影響。

實驗結果顯示枯草桿菌於碳氮比20組，生長顯著高於控制組 (p < 0.05)；添加碳源的處理組，皆有效提升光合菌生長，與控制組有顯著差異 (p < 0.05)，其中以碳氮比15組有最高菌量；副溶血弧菌於碳氮比5組生長較快，與控制組有顯著差異 (p < 0.05)。水質處理的結果顯示枯草桿菌在各處理組皆能降低氨-氮濃度，各組間無顯著差異 (p > 0.05)，亞硝酸-氮濃度於碳氮比20組顯著降低，與控制組有顯著差異 (p < 0.05)；光合菌於添加碳源的組別，氨-氮與亞硝酸-氮濃度皆下降，其中以碳氮比10、15、20組，水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05)；副溶血弧菌於碳氮比15、20組，水中氨-氮與亞硝酸-氮濃度顯著低於控制組 (p < 0.05)。抑制實驗中枯草桿菌103、105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml有抑制生長效果，光合菌105 cfu/ ml添加量對於副溶血弧菌103 cfu/ ml，第72小時後有抑制生長效果。

添加碳源提高水體中碳氮比，能有效促進水中細菌對水質的改善，並且營造較適合枯草桿菌和光合菌的環境，達到抑制副溶血弧菌生長。

關鍵字

碳氮比 ； 枯草桿菌 ； 光合菌 ； 副溶血弧菌 ； 生長 ； 水質 ； 抑制

並列摘要

This study added molasses as carbon source into the water to control carbon/ nitrogen ratios at five levels, 0, 5, 10, 15 and 20. 0 added nothing as a control group. To investigate different carbon/ nitrogen ratios effects of Bacillus subtilis, Rhodobacter sphaeroides and Vibrio parahaemolyticus on growth, water quality and mutual inhibition.

In the experiment of the bacteria growth in different carbon/ nitrogen ratios water, the results show that Bacillus subtilis at 20 group were significantly higher than the control (p < 0.05). Each treatment group increased growth of Rhodobacter sphaeroides significantly compared to the control group (p < 0.05), while 15 treatment group had better effect than all treatment group. In addition, Vibrio parahaemolyticus at 5 treatment group were significantly increased rate of growth to control (p < 0.05). In the experiment of the water quality of bacteria in different carbon/ nitrogen ratios water, the results indicated that Bacillus subtilis reduced ammonia-N concentrations in water, but it was not effects significantly on each treatment group. In addition, the nitrite-N concentrations significantly lower at 20 treatment group than control (p < 0.05). Each treatment group of Rhodobacter sphaeroides, which reduced ammonia-N and nitrite-N concentration significantly compared to the control group (p < 0.05), while 10、15 and 20 treatment had better results. Vibrio parahaemolyticus at 15、20 treatment reduce the ammonia-N and nitrite-N concentrations significantly than control group (p < 0.05). Through the inhibition of bacteria growth, 103、105 cfu/ ml of Bacillus subtilis can produce inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus. The 105 cfu/ ml of Rhodobacter sphaeroides had inhibition effects on 103 cfu/ ml Vibrio parahaemolyticus after 72 hr.

In conclusion of this study, carbon source addition increase carbon/ nitrogen ratios in water can effectively improve the water quality and growth activities of Bacillus subtilis and Rhodobacter sphaeroides. To build a suitable environment of probiotic for more efficiently inhibit the growth of Vibrio parahaemolyticus.

並列關鍵字

Carbon/ Nitrogen Ratios ； Bacillus subtilis ； Rhodobacter sphaeroides ； Vibrio parahaemolyticus ； Growth ； Water quality ； Inhibition

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