探討 sodium butyrate 在病毒防治之利用與機轉

Investigation of the effect of sodium butyrate on plant virus prevention and its mechanism

李佩穎(Pei-ying Li)

指導教授 ： 張雅君

共同指導教授 ： 葉信宏(Hsin-Hung Yeh)

國立臺灣大學/生物資源暨農學院/植物病理與微生物學研究所/碩士(2014年)

https://doi.org/10.6342/NTU.2014.01051

摘要

病毒於感染植物之後，常造成植株無法復原的傷害，而穩定有效的病毒防治策略仍有待發展。Histone deacetylases (HDACs) 在細胞中負責調控 histone 修飾行為，藉以影響基因的表現。過去研究顯示 Brome mosaic virus, Tomato bushy stunt virus 此兩種 RNA 病毒在 HDACs 基因功能缺失的酵母菌中，其病毒的累積會受到影響而下降，且 Histone deacetylase inhibitors (HDACi) 更被發現在 HCV replicon cell OR6上對C型肝炎病毒的複製有顯著的抑制效果，但這類物質的作用卻缺乏植物病毒方面的探討，因此本實驗選用sodium butyrate (HDACi 中的一種) 在易感病圓葉菸草進行菸草嵌紋病毒抗性測試。實驗結果顯示 sodium butyrate 雖無法阻止病毒系統性感染植株，但可延遲病株的病程發展並減緩外部病徵，且在抗性蛋白的時程表現上，有測得水楊酸抗性途徑之標記基因 PR-1a 的生成，推測其可能的調控與水楊酸路徑相關，且其在系統葉上的表現量較處理葉為多，但相反地水楊酸處理的植株，其處理葉上的 PR-1a 其表現量比系統葉來得多，顯示sodium butyrate誘導系統性抗性較水楊酸為佳，但局部抗性誘導以水楊酸為佳，因此將水楊酸和 sodium butyrate 合併塗抹於葉片上，實驗結果顯示，合併使用其抗性有加成效果。另外在活性氧物質的測定上，1 mM 的sodium butyrate單獨使用處理也會造成系統葉上過氧化氫的累積。因此 sodium butyrate 的誘導系統性抗病能力應為未來可應用之方法，期為植物病毒病害防治提供新的方向。

關鍵字

histone deacetylases (HDACs) ； histone deacetylase inhibitors (HDACi) ； sodium butyrate ； 菸草嵌紋病毒 ； 圓葉菸草

並列摘要

Viruses cause serious damage to crops; however, effective plant antiviral disease managements remain largely to be explored. Histone deacetylases (HDACs) are genes involved in histone regulation, and modulate the expression of genes. Previous studies demonstrated that in histone deacetylases (HDACs) single-gene-knockout yeasts the accumulation of Brome mosaic virus and Tomato bushy stunt virus decreased, and application of histone deacetylase inhibitors (HDACi) to HCV replicon cell OR6 showed suppressive effect on Hepatitis C virus. However, whether HDACi can be applied for plant viral disease management remained to be resolved. In this study, we first treated sodium butyrate (one kind of HDACi) to Nicotiana benthamiana, a susceptible host, to Tobacco mosaic virus (TMV). The data indicated that plants pretreated with sodium butyrate showed more vigorously growth and delayed in symptom expression than untreated plants after TMV inoculation. In addition, our RT-PCR revealed that the application of sodium butyrate induced the expression of PR-1a, suggested that sodium butyrate participated in the salicylic acids (SA) related plant defense pathway. Furthermore, in contrast to SA-treated plants the expression of PR-1a is stronger in treated leaves than systemic leaves; sodium butyrate-treated leaf shows stronger PR-1a induction in systemic leaves. The production of ROS showed obviously increase in the systemic leaf of sodium butyrate-treated N. benthamiana. It indicated that SA may trigger stronger local defense and sodium butyrate trigger stronger systemic defense. Thus, we applied both SA and sodium butyrate solutions on the leaves of N. benthamiana, and enhanced resistance was observed on the mixed solutions-treated plants. It revealed that sodium butyrate has the potential to be applied in development of effective systemic antiviral disease managements.

並列關鍵字

histone deacetylases (HDACs) ； histone deacetylase inhibitors (HDACi) ； sodium butyrate ； TMV ； Nicotiana benthamiana

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