以電紡絲法製備聚己內酯/動物明膠 同軸奈米纖維及其應用

Fabrication and Application of Polycaprolactone/Gelatin Coaxial Nanofibers by Electrospinning

周芳伃(Fang-Yu Chou)

指導教授 ： 謝學真

國立臺灣大學/工學院/化學工程學研究所/碩士(2015年)

https://doi.org/10.6342/NTU.2015.10444

摘要

同軸靜電紡絲法可用於製備具有核/殼層結構(core/shell)的同軸奈米纖維絲(coaxial nanofibers)，例如可將機械強度高之材料置於核層(core layer)，生物相容性高之材料置於殼層(shell layer)，以製造同時擁有好的機械強度及生物相容性之支架。另外，亦可將不穩定的材料置於核層以達到保護的作用，或是將藥物放於核層以達到穩定藥物釋放的效果。本研究選擇以動物明膠(gelatin)置於殼層以增加材料之親水性；聚己內酯(polycaprolacton，簡稱PCL)置於核層以增加材料之穩定性及機械強度，預期能製備具核殼結構的同軸奈米纖維膜，應用於延緩藥物的釋放。本研究固定核層聚己內酯之濃度及流量，改變殼層濃度及流量，搭配不同電壓進行電紡，再以掃描式電子顯微鏡(SEM)及穿透式電子顯微鏡(TEM)觀察同軸纖維之型態與結構，最後選出殼層之動物明膠濃度為20%，操作電壓為25 kV，核/殼層流量比為1:2的操作條件下，可製備有良好核/殼層結構之同軸奈米纖維。
本研究再以紅外線吸收光譜(FTIR)測定，驗證聚己內酯及動物明膠皆存在於同軸奈米纖維內；由接觸角測定結果可知聚己內酯/動物明膠(簡稱P/G)同軸奈米纖維膜擁有較高之親水性，並且在以戊二醛(glutaraldehyde)交聯後，其機械強度和延展性有明顯的提升；另外在崩解性質測定結果中，可以得知在交聯後明顯提升P/G同軸奈米纖維之材料穩定性；由機械強度測試結果可知交聯過後之同軸奈米纖維，相較於單成分的動物明膠纖維膜，擁有較好的機械強度；在藥物釋放測試方面，經過戊二醛蒸氣交聯兩小時之同軸奈米纖維膜最可以延緩薑黃素釋放之速率。
由於交聯後之P/G同軸奈米纖維具有較高之機械強度及延緩藥物釋放的效果，再加上較佳之親水性可增進細胞貼附，因此是非常具有潛力的生醫材料。

關鍵字

同軸奈米纖維 ； 靜電紡絲法 ； 聚己內酯 ； 動物明膠 ； 核/殼結構 ； 藥物釋放

並列摘要

Electrospinning can be used to produce coaxial nanofibers with core/shell structure. For example, tissue engineering scaffolds with enhanced functionality can be created by coaxial electrospinning using a biocompatible material as the shell layer and a high-strength material as the core layer. Unstable materials can be loaded into the core layer and thus protected by the shell layer. Coaxial nanofibers can be also used to prolong drug release if the drug is placed in the core layer.
In this study, coaxial nanofibers with polycaprolactone (PCL) and gelatin as the core and shell layers were prepared for controlled drug release. PCL provides mechanical strength and gelatin increases surface hydrophilicity of the nanofibers. The coaxial electrospinning was carried out under various conditions. Scanning electron microscope (SEM) and transmission electron microscopy (TEM) were used to observe the structure and morphology of the coaxial nanofibers and then determine the optimum parameters for electrospinning. The morphology of the PCL/gelatin (core/shell) coaxial nanofibers was satisfactory when the concentration of PCL solution was 15 wt%, with the concentration of gelatin solution being 20 wt%, and the flow rate ratio of core to shell solution being 1:2.
Furthermore, the Fourier Transform Infrared (FTIR) assay confirmed the presence of PCL and gelatin in the coaxial nanofibers. The water contact angle measurements revealed that the PCL/gelatin coaxial nanofibers were more hydrophilic than the PCL nanofibers. The degradation experiments indicated that after cross-linking by glutaraldehyde the stability of the coaxial nanofibers was enhanced significantly. As compared with the gelatin nanofibers, the mechanical strength of the PCL/gelatin coaxial nanofibers was greater especially after cross-linking. In the drug release experiments, the cross-linked coaxial nanofibers were able to release the drug (curcumin) in a steady and sustained manner.
The PCL/gelatin coaxial nanofibers possess good mechanical strength, steady drug release behavior, and improved hydrophilicity. Hence, the prepared PCL/gelatin coaxial nanofibers have vast potential in the future.

並列關鍵字

Coaxial nanofibers ； Electrospinning ； Polycaprolacton ； Gelatin ； Core-shell structure ； Drug release

參考文獻

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