左旋維他命C-蒙脫石奈米黏土複合物:合成與毒性研究

L-ascorbic acid-montmorillonite Nanocomposite: Synthesis and Toxicity

林武慶(Wu-Ching Lin)

指導教授 ： 李源弘

共同指導教授 ： 陳博彥

國立臺灣大學/工學院/材料科學與工程學研究所/碩士(2005年)

https://doi.org/10.6342/NTU.2005.00610

摘要

本研究利用市面上最熱門的左旋維他命C來探討其和蒙脫石複合以後，對左旋維他命C安定性和毒性測試，大多數對於左旋維他命C（L-ascorbic acid）之相關研究，多是以高分子劑型方式來開發，藉由利用蒙脫石特有之層間特性，探討出合適左旋維他命C-蒙脫石複合物，並試圖找出左旋維他命C插入或吸附於蒙脫石之最佳條件，再經由SEM、XRD、FT-IR光譜解析，深入探討蒙脫石之插入行為，並以麵包酵母菌為指標針對複合物做毒性測試，比較蒙脫石/左旋C複合物、蒙脫石、左旋C三者之劑量響應關係，就EC50而言，蒙脫石/左旋C複合物毒性是最小，EC0方面，蒙脫石/左旋C複合物之抗菌力是最佳的，綜合以上結果，以利後續進行載體控制釋放之可行性分析。
先前研究中發現，蒙脫石（montmorillonite）天然複合物噴灑於九層塔植物葉片上具有防止葉片黃化及間接防治蟲害之作用。為深入瞭解其機制（如：施灑後之殘留毒性），本研究取定指標微生物之枯草菌（Bacillus subtilis CCRC 14714）來進行生物毒性分析。利用蒙脫石之各種配方複合物組合為介質來監測其微生物生長反應行為，並定義出劑量-響應曲線求出有效劑量值（例如：EC0, EC50），後再與典型農藥（如Lannate , C5H10N2O2S）作毒性對照比較，另一方面，枯草菌與麵包酵母菌之劑量響應曲線作對照，觀察兩者之類比平行毒性關係。

關鍵字

蒙脫石 ； 黑色素 ； 枯草菌 ； 麵包酵母菌 ； 劑量-響應分析 ； Lannate ； 左旋維他命C

並列摘要

In this study, we used the famous cosmetics “L-Ascorbic acid” to mix with montmorillonite, and then observed the stability of L-ascorbic acid and tested the toxicity. Recently, many people have studied on the L-ascorbic acid, but most of them are developed with polymer type. By using the laminar property of montnorillonite, we can synthesize the proper L-ascorbic acid/montmorillonite composites. However, we can try to find the best formula for the intercalation of the L-ascorbic acid, and then experiment with SEM, XRD and FT-IR. After we understood the intercalation, we tested the toxicity of the hybrid (montmorillonite/L-ascorbic acid, montmorillonite, L-ascorbic acid) for Saccharomyces cerevisiae by dose-response analysis. The toxicity of montmorillonite/L-ascorbic acid is the lowest for EC50, and the anti-bacterium of montmorillonite/L-ascorbic acid is best for EC0. To sum up, it could be feasible to use the montmorillonite as the drug-carrier.
As indicated previously, montmorillonite composites sprayed on Qcimum basilicum L. leafage could prevent leaves from opaque yellow and indirectly keep the worm away. To understand the mechanism, we used, Bacillus subtilis, to be our experimental model to analyze the toxicity. We administrated with different doses of montmorillonite to observe the growth behavior of microorganism. After that, we defined the dose-response curve (EC50, EC0 e.t.c.), and compared with typical pesticide (ex. Lannate). On the other hand, we compared the dose-reponse curves of Bacillus subtilis with Saccharomyces cerevisiae, and observed the parallel toxic relationship.

並列關鍵字

Montmorillonite ； Melanin ； Bacillus subtilis ； Saccharomyces cerevisiae ； Dose-response analysis ； L-ascorbic acid

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