利用毛細管區帶電泳法藉由添加對掌解析試劑以分離對掌異構物是一種有效的分析技術。本論文將以此法為主,針對五種抗組織胺對掌異構物之遷移及分離行為予以研究,內容分為兩部份: 第一部份是探討將以添加中性環糊精作為對掌解析試劑時所觀察到分析物的分離及遷移行為。並藉由電泳遷移率之變化趨勢配合電腦數值模擬求得分析物與環糊精之結合常數。由分析物的電泳遷移率對環糊精濃度作圖,發現分析物電泳遷移率的趨勢隨著添加環糊精濃度的改變有非常明顯變化,顯示分析物與環糊精之間具有很強的結合作用力。經由實驗點的曲線模擬可以求出分析物與環糊精之間的結合常數,結合常數的量值關係著兩者之間作用力的大小,應用在電泳分離過程中,能夠預測添加環糊精的濃度對分析物遷移行為的影響,進而推測最佳的分離條件。 第二部份藉由添加帶電荷的硫酸鹽衍生化環糊精[single-isomer heptakis (2,3-dihydroxy-6-O-sulfo)-β-cyclodextrin (SI-S-β-CD)與randomly sulfate-substituted β-CD (MI-S-β-CD)] 作為對掌解析試劑,對五種具對掌異構物之分析物予以分離,尋求在此種操作模式下之最佳分離條件。此外也嘗試混合兩種帶電荷的硫酸鹽衍生化環糊精來增加對掌分離的效果。從實驗結果發現,在混合環糊精的方式下可以將五種分析物的對掌異構物予以分離,而且其中幾種分析物相較於單純加添SI-S-β-CD硫酸鹽衍生化環糊精時會具有加乘分離的效果。
Capillary zone electrophoresis has proven to be a powerful technique for enantioseparation. In this study, five chiral antihistamines were investigated using cyclodextrins as chiral selectors. The results of this thesis are presented in two parts: In the first part, we focus on the binding constants of antihistamines with addition of neutral cyclodextrins (HP-β-CD, β-CD, DM-β-CD). In this work, the interaction of chiral analytes with chiral selectors was studied through the variation of mobility as a function of neutral cyclodextrins concentration. Binding constants of antihistamines to HP-β-CD, β-CD and DM-β-CD were evaluated through curve-fitting. The results indicate that the interaction of antihistamines with neutral cyclodextrins, which are also affected by antihistamines structures, play a major role in the determination of enantioseparation. In the second part , we focus on the enantioseparation of antihistamines with addition of randomly sulfate-substituted β-CD (MI-S-β-CD) and single-isomer heptakis (2,3-dihydroxy-6-O-sulfo )-β-cyclodextrin (SI-S-β-CD) and the optimization of separation. Moreover, by mixing MI-S-β-CD with SI-S-β-CD, the enantioseparation efficiency is considerably enhanced. In comparison with the use of SI-S-β-CD alone, the enantiomers of three antihistamines could be baseline separated when mixed cyclodextrins were used.