以硫酸鹽衍生化環糊精為對掌選擇劑對兒茶酚胺類 對掌異構物分離之研究

Enantioseparation of Catecholamines and Structurally Related Compounds by Capillary Zone Electrophoresis Using Sulfated-β-Cyclodextrin as Chiral Selectors.

鄭旭敦(Hsu-Tun Cheng)

指導教授 ： 林敬二

國立臺灣大學/理學院/化學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.00701

摘要

本篇論文利用毛細管區帶電泳法藉由添加對掌選擇劑以分離對掌異構物，針對三種兒茶酚胺類及三種結構相似物種之對掌異構物進行對掌異構物分離，並研究其遷移行為及探討分離的最佳化條件。首先是藉由添加帶電荷的硫酸鹽衍生化環糊精來分離這六種具對掌異構物之分析物，進行改變各項分離參數來找尋最佳化之分離條件。並嘗試添加其他種類的環糊精來進行對掌分離。藉由不同種類環糊精對這些對掌異構物的分離，了解環糊精對分析物及分析物對掌異構物的分離機制。另一方面，利用雙環糊精系統來分離兒茶酚胺類化合物，研究這些分析物在電泳過程中的遷移行為以及探討化合物對掌異構物的遷移次序反轉現象及其遷移次序反轉的源由。

關鍵字

兒茶酚 ； 胺對掌異構物 ； 對掌分離 ； 環糊精

並列摘要

In this study, we focus on the enantioseparation of three catecholamines and three structurally related compounds with addition of sulfated cyclodextrins and the optimization of separation. The influences of buffer concentration and temperature on the migration behavior and enantioseparation of these cationic solutes with addition of sulfated cyclodextrins were investigated. The results indicate that, buffer concentration and temperature play important roles in the enantioseparation of these cationic solutes. In order to understand enantoseparation mechanisms of these cationic solutes also used three kind of neutral cyclodextrins as chiral selectors. Moreover, by mixing two kind of cyclodextrins, we found the reversal of migration order of norephedrine、synephrine and octopamine. The interaction of chiral analytes with chiral selectors was studied through the variation of mobility of norephedrine、synephrine and octopamine.

並列關鍵字

Catecholamine ； chiral separation ； cyclodextrin ； enant

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