- 學位論文
毛細電泳電灑法質譜於胺基醣苷類抗生素之分析
The analysis of aminoglycosides using capillary electrophoresis/electrospray ionization mass spectrometry
摘要
毛細管電泳電灑法質譜是近代備受矚目及研究的一項分析技術，主要原因為毛細管電泳擁有高理論板數的特性，且能藉由各分析物離子在電場中依其電泳淌度的差異造成遷移速率不同而達到優異的分離效果，因此毛細管電泳電灑法質譜至今被廣泛應用於各分析領域，如食品、醫藥、生物、環境等。胺基醣苷為一種抗生素類化合物，常被過度使用在肉類製品及乳製品中且有殘留的問題，對人類健康形成危害。 目前大多數的文獻均使用液相層析來分析食品中之胺基醣苷類化合物，然而離子對試劑的添加常會對分析物的離子化效果及訊號感度造成影響，毛細管電泳相較於液相層析來說較不會有訊號抑制的情形。因此本研究利用醋酸銨溶液在低pH值的環境下，得到優異的分離效果，操作在先前實驗室已開發的低流速鞘流介面銜接毛細管電泳電灑法質譜於選擇反應偵測模式下 ( Selected Reaction Monitoring ) 進行掃描以提高分析物訊號之靈敏度，搭配固相萃取前處理方法來分析牛奶樣品中七種胺基醣苷類抗生素。偵測極限約落在2.5~10 ppb之間。
並列摘要
CE-ESI/MS is a widely used analytical method in many fields and environment due to its high sensitivity and selectivity. Aminoglycoside is an antibiotic for animal husbandry, however, overuse of aminoglycoside is a serious issue in human health. The analysis of aminoglycoside is usually performed by LC-MS with ion pairing reagent. However, the addition of ion pairing reagent would cause signal suppression. Compared with LC-MS, the ion pairing reagent is not required for CE-MS. In this study, MS was operated at SRM mode with a low flow interface under low pH ammonium acetate buffer solution. The detection limit was 2.5 – 10 ppb for aminoglycosides in milk prepared by SPE method.